ヒト歯根膜細胞の細胞外基質蛋白質の発現の特徴について

• 笹栗 健一

  神奈川歯科大学歯科矯正学教室

書誌事項

タイトル別名

• The gene expressions of extracellular matrix molecules in human periodontal ligament fibroblast(HPLF).

抄録

It has been reported that bone type alkaline phosphatase (ALP) has a higher activity in human periodontal ligament fibroblasts (HPLF) than that of other soft connective tissue cells, such as gingiva and skin 6broblasts.Since the ALP activity of HPLF is markedly stimulated by 1, 25 (OH) ₂D₃, which incidentally is one of the characters of osteoblasts, it was suggested that HPLF is an osteoblastic. fi broblast. These findings led us to the further characterization of osteoblastic features of HPLF in order to mderstand the physiological function of HPLF.<BR>In this study, the anchorage-independent colony formation assay, biosynthesis of secreted phosphoproteins and gene expression using specific probes (cDNA) of TGF-β, liver/bcne/kidney ALP, 2ar/osteopontin and SPARC/osteonectin were investigated. Human gingival cells (Gin-1), human pulp clone cell (593C1) and rat osteoblastic cells (A₁₁, ROS17/2.8) were used to compare with HPLF.<BR>The findings were as follows: conditioned media from HPLF were able to induce colony formation similar to that of A₁₁ and ROS17/2.8, indicating that HPLF secreted a TGF-β-like factor. The radio-labeled osteopontins were synthesized with Mrs.45K and 67K in ROS17/2.8, while the proteins were not detected in HPLF. Finally, a higher expression of mRNAs of ALP, TGF-βand SPARC mRNA were revealed in HPLF by the northern blot analysis, but no 2ar mRNA was detected.<BR>In conclusion, these results indicate that HPLF presents osteoblastic characters based on the gene expression of ALP, TGF-βand SPARC. However, the biosynthesis of phosphoprotein was found to be distinct from osteoblastic cells.

収録刊行物

• 歯科基礎医学会雑誌

  歯科基礎医学会雑誌 33 (3), 261-274, 1991

  歯科基礎医学会

キーワード

• Human Periodontal ligament fibroblast
• transforming growth factor-β
• alkaline phosphatase
• osteopontin
• osteonectin

詳細情報

• CRID: 1390001204682765440
• NII論文ID: 130004001668
• DOI: 10.2330/joralbiosci1965.33.261
• ISSN: 03850137
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可