親水‐疎水二層構造をもつ多孔質ポリテトラフルオロエチレン膜を用いた酵素電極の作製

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タイトル別名
  • Employment of an Asymmetric Porous Poly (tetrafluoroethylene) Membrane for Construction of an Ammonia-Electrode-Based Enzyme Electrode
  • シンスイ ソスイ 2ソウ コウゾウ オ モツ タコウシツ ポリテトラフルオロエ

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抄録

A novel asymmetric porous poly (tetrafluoroethylene) membrane (pore size 0.3 μm, thickness 70 μm) possessing a hydrophilic (thickness 30 μm) and hydrophobic (thickness 40 μm) stratal structure was employed to construct an ammonia-electrode-based adenosine electrode. Adenosine deaminase was immobilized in the micropores of the hydrophilic part of the membrane, thus leading to protection of the mechanically weak enzyme layer by the robust poly (tetrafluoroethylene) matrix. The hydrophobic part of the membrane acted as a gas-permeable membrane. Therefore, an adenosine electrode could be constructed by using only a single membrane and a pH electrode. Advantages of employing the enzyme-immobilized asymmetric membrane are: simpler procedures of electrode construction and fast response time, e. g., the time required to attain steady E. M. F. was 2 min. at 10-4 mol/l adenosine at pH =8.5 and at 25°C, while that for a conventional electrode using two separate membrane was 27 min [I. Deng, et al., Anal. Chem., 52, 1937 (1980)] .

収録刊行物

  • 高分子論文集

    高分子論文集 41 (4), 229-232, 1984

    公益社団法人 高分子学会

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