Nα-Acetyltransferase NatA Is Involved in Ribosome Synthesis in Saccharomyces cerevisiae

• WAN Kun

  Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University

• TSUCHIHASHI Kanako

  Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University

• KANDA Kazuhiro

  Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University

• SHIMOJI Kaori

  Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University

• MIZUTA Keiko

  Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University

書誌事項

タイトル別名

• <i>N</i>^α-Acetyltransferase NatA Is Involved in Ribosome Synthesis in <i>Saccharomyces cerevisiae</i>

抄録

Ebp2 has an essential role in the biogenesis of 60S ribosomal subunits. Synthetic-sick alleles with the ebp2-14 mutation were screened. The mutations were localized to the ARD1 and NAT1 genes, which encode the catalytic subunit and the auxiliary subunit of N^α-acetyltransferase NatA respectively. Polysome analyses revealed that ard1Δ and nat1Δ caused a synergistic defect with ebp2-14 in the assembly of 60S ribosomal subunits. To identify the proteins that functionally interact with NatA, we designed mutants in which the second amino acid was substituted for proline in Ebp2 and functionally related proteins: Brx1, a partner of Ebp2 in ribosome biogenesis, and the ribosomal protein L36a/b, overexpression of which suppresses a growth defect in ebp2-14. Among these, only brx1-S2P exhibited a synthetic defect with ebp2-14. These results suggest that optimal NatA function is important to the cooperative function of Brx1 with Ebp2 in 60S ribosomal subunit biogenesis.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (3), 631-638, 2013

  公益社団法人 日本農芸化学会

キーワード

• ribosome biogenesis
• <i>N</i>^α-acetylation
• NatA
• ribosomal protein L36
• Ebp2

詳細情報

• CRID: 1390282681456023552
• NII論文ID: 10031164787
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.120860
• COI: 1:STN:280:DC%2BC3svkt1Cnsw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024391242
• PubMed: 23470771
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024391242
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.120860
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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