Inter-sectional hybrids obtained from reciprocal crosses between Begonia semperflorens (section Begonia) and B. 'Orange Rubra' (section Gaerdita×section Pritzelia)

• Chen Yen-Ming

  Laboratory of Plant Cell Technology, Faculty of Horticulture, Chiba University

• Mii Masahiro

  Laboratory of Plant Cell Technology, Faculty of Horticulture, Chiba University

書誌事項

タイトル別名

• Inter-sectional hybrids obtained from reciprocal crosses between <i>Begonia semperflorens</i> (section <i>Begonia</i>) and <i>B.</i> ‘Orange Rubra’ (section <i>Gaerdita</i> × section <i>Pritzelia</i>)

抄録

Inter-sectional hybrids were successfully obtained by the reciprocal crosses between 11 cultivars (including 6 diploids and 5 tetraploids) of Begonia semperflorens (SS & SSSS genomes) and B. ‘Orange Rubra’ (RR genome) with the aid of in vitro culture of mature or immature seeds on MS medium containing 0.1 mg l^{<symbol>-</symbol>1} α-naphthylacetic acid, 0.1 mg l^{<symbol>-</symbol>1} 6-benzyladenine, 10 mg l^{<symbol>-</symbol>1} gibberellic acid, 30 g l^{<symbol>-</symbol>1} sucrose and 2.5 g l^{<symbol>-</symbol>1} gellan gum. Embryo rescue as ovary culture with immature seeds 12^th–16^th day after pollination (DAP) generally gave higher efficiency of plantlet formation, but in some cross combinations, culture of mature seeds (30 DAP) resulted in higher yield of plantlets. Flow cytometric analysis revealed that they were consisted of the plants with various genomic combinations (RS, RR, RSS, RRS, RRSS and RRRRSS) as estimated by the DNA contents of both parents. Hybridity of these plants with various genomic combinations including RR was confirmed by random amplified polymorphic DNA analysis. These results suggested that unreduced gamete formation and spontaneous chromosome doubling were involved in the hybrid formation of various ploidy levels and genomic combinations. These hybrids showed various levels of intermediate traits between both parents according to the genomic compositions, and some of them had desirable characters of both parents.<br>

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 62 (2), 113-123, 2012

  日本育種学会

キーワード

• amphipolyploid
• <i>Begonia</i> ‘Orange Rubra’
• <i>Begonia semperflorens</i>
• embryo rescue
• inter-sectional hybrid
• unreduced gamete

詳細情報

• CRID: 1390001204724173952
• NII論文ID: 130004057149
• NII論文ID: 10030434467
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.62.113
• COI: 1:CAS:528:DC%2BC38XhsFSjtL%2FO
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 023738712
• PubMed: 23136522
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023738712
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/62/2/62_113/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可