マウスはい発生能に及ぼす体細胞との共培養の効果とはい発育促進因子同定の試み

• 廣瀬 雅哉

  滋賀医科大学産科学婦人科学教室

• 喜多 伸幸

  滋賀医科大学産科学婦人科学教室

• 石 紅

  滋賀医科大学産科学婦人科学教室

• 高倉 賢二

  滋賀医科大学産科学婦人科学教室

• 野田 洋一

  滋賀医科大学産科学婦人科学教室

• 岡部 英俊

  滋賀医科大学臨床検査科

書誌事項

タイトル別名

• Embryotropic factor to mouse preimplantation embryo in coculture with somatic cells.

抄録

Growth of mouse preimplantation embryos in several kinds of coculture with somatic cells was observed and related embryotropic factors were investigated. Mouse pronuclear stage embryos were cocultured with rabbit oviductal cells, rabbit endometrial epithelial cells, human dermatofibrosarcoma cells, human liposarcoma cells, guinea-pig intraperitoneal macrophages, or mouse intraperitoneal macrophages, respectively. Mouse embryos grew in coculture with rabbit oviductal cells the same as in basal medium (control). Embryos cocultured with rabbit endometrial epithelial cells, human dermatofibrosarcoma cells or human liposarcoma cells showed significantly worse development than the control. Coculture with guinea-pig intraperitoneal macrophages or mouse intraperitoneal macrophages significantly enhanced embryonic development. Although the number of cells comprising the embryos significantly increased in coculture with MO, these were less than those of in-vivo embryos. Decrease of the number of cells of MO coexisting with embryos reduced the enhancing effect of embryonic development in coculture. Prolongation of time between harvest of MO and coculture brought less embryotropic effect. Embryos showed worse growth in conditioned medium of MO than the control. Although twenty five amino acids were detected in the conditioning medium with MO, the amounts were very low. In analysis with antibodies against human or rat materials, IL-1Q was 100 pg/ml and other cytokines or growth factors (IL-1a, IL-1/3, IL-6, TNF-a, IFN-a, TGF-a, TGF-f, IGF- I, IGF-II, EGF, PDGF-AB, PDGF-BB, GM-CSF, G-CSF) were undetectable. Prostanoids in the conditioned medium were quantified as follows; PGF2a: 325.0 pg/ml, PGE2: 635.0pg/ml, 6keto-PGF, a: 235. Opg/ml, TXB2: 2180.0pg/ml. Indomethacin had no influence upon coculture with M. Coculture with Mck did not improve the implantation rate in transfer to pseudopregnant mice. In conclusion, coculture with guinea-pig intraperitoneal macrophages improved embryonic development with the effect being thought to be brought about by an unstable factor other than cytokines, growth factors and prostanoids. The implantation rate of cocultured embryos was very low in spite of morphological improvement. It was suggested that although a system improved embryonic development morphologically, as did our coculture system, the pregnancy rate may not necessarily accelerate. [Adv Obstet Gynecol 49(5); 528-542, 1997 (H9.9)]

収録刊行物

• 産婦人科の進歩

  産婦人科の進歩 49 (5), 528-542, 1997

  近畿産科婦人科学会

キーワード

• mouse embryo
• coculture
• macrophage implantation

詳細情報

• CRID: 1390282679924413824
• NII論文ID: 130004069597
• DOI: 10.11437/sanpunosinpo1949.49.528
• ISSN: 13476742
• ISSN: 03708446
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可