血液分画製剤に関する品質試験　アンチトロンビンの性状分析

• 石井 良和

  長崎大学医学部附属病院薬剤部

• 馬場 幸

  長崎大学医学部附属病院薬剤部

• 市川 正孝

  長崎大学医学部附属病院薬剤部

書誌事項

タイトル別名

• Quality Control of Blood Derivatives: Properties of Concentrated Products of Human Antithrombin. III.
• Properties of Concentrated Products of Human Antithrombin III
• アンチトロンビンの性状分析

抄録

Human antithrombin III (AT III) products have been developed by two companies, Green Cross Co.Ltd.and Hoechst Japan in Japan.These two companies have recently modified the purification methods for AT III.New products from Green Cross and Hoechst Japan had 1.7-f old and 1.9-fold higher activities than the old type products, respectively.A single band was observed by SDS-polyacrylamide gel electrophoresis, and its molecular weight was evaluated to be about 63, 000 daltons.Two bands were detected by isoelectric focusing, and the isoelectric point of the major band was about 5.10.On the other hand, two peaks were observed in the old AT III product from Green Cross by use of high-performance liquid chromatography.The molecular weight of the major peak was approximately 60, 000 daltons, while that of the minor peak was about 159, 000 daltons.The precipitation of the AT III products indicated completeidentity by the double immunodiffusion assay.From these results, the quality of the new AT III products from both manufacturers were confirmed to be improved.

収録刊行物

• 病院薬学

  病院薬学 18 (3), 299-303, 1992

  一般社団法人 日本医療薬学会

キーワード

• qualitative test
• quality control of blood derivative
• antithrombin III products
• biological property of antithrombin III

詳細情報

• CRID: 1390282679751964800
• NII論文ID: 130004103083
• DOI: 10.5649/jjphcs1975.18.299
• ISSN: 21859477
• ISSN: 03899098
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可