免疫比ろう法に基づく梅毒Ｔｒｅｐｏｎｅｍａ　Ｐａｌｌｉｄｕｍ抗体測定自動化の試み

• 原 喜与一

  国立療養所兵庫中央病院研究検査科

• 扇谷 茂樹

  国立療養所兵庫中央病院研究検査科

書誌事項

タイトル別名

• Automatic Analysis Using Nephelometric Method for Serum Treponema Pallidum Antibody.

抄録

Among the tests in which T. pallidum is used as antigen, T. pallidum hemagglutination (TPHA) test and fluorescent treponemal antibody-absorption (FTA-ABS) are most commonly used for clinical serodiagnosis.<br>These tests need a complicated pretreatment of the sera (semi-quantitative method) as well as requiring special techniques, thus increasing the reaction time and affecting intra-and interassay precision studies unfavorable.<br>To overcome these problems, we evaluated latex agglutination test for T. Pallidum (TPLA) with Behring Nephelometer Analyzar (BNA).<br>The linearity of the TPLA ranged from 40 to 560 titer units (T. U.). Intra-and interassay pre-cision studies showed high reproducibility. Protentially interfering substances, such as bilirubin, hemoglobin and rheumatoid factor, did not affect the result.<br>There was a significant correlation between TPLA and TPHA (n=67, γ=0.85, p<0.001). The agreement between TPLA and TPHA for positive sera was 91%. Result of intra-and interassay, correlation with TPHA and the short reaction time (12 min compared to 2 hr for TPHA ) make TPLA a useful candidate for screening of serum treponemal antibody.

収録刊行物

• 医療

  医療 51 (3), 106-110, 1997

  一般社団法人 国立医療学会

キーワード

• 抗Treponema Pallidum 抗体
• TPHA法
• TPLA法
• ラテックス凝集法
• ネフェロメトリー法

詳細情報

• CRID: 1390282681289610624
• NII論文ID: 130004107608
• DOI: 10.11261/iryo1946.51.106
• ISSN: 18848729
• ISSN: 00211699
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可