Construction of an Endogenous Selectable Marker Gene for the Rice Straw Degrading White-Rot Basidiomycete Cyathus stercoreus

• YAMAGISHI Kenji

  NARO Tohoku Agricultural Research Center

• KIMURA Toshiyuki

  NARO Tohoku Agricultural Research Center

• WATANABE Takashi

  Laboratory for Biomass Conversion, Research Institute for a Sustainable Humanosphere, Kyoto University

書誌事項

タイトル別名

• Construction of an Endogenous Selectable Marker Gene for the Rice Straw Degrading White-Rot Basidiomycete <i>Cyathus stercoreus</i>

抄録

Cyathus stercoreus has a superior ability to increase the cellulase saccharification yields of rice straw under biological pretreatment. As a first step to improve the biological pretreatment efficiency of C. stercoreus further, a new gene transformation system was constructed. Uracil auxotrophic mutant uv-180 was generated as host strain by ultraviolet radiation. It was transformed using plasmid pGE containing the orotate phosphoribosyltransferase (CsURA5) and enhanced green fluorescent protein (egfp). Many transformants exhibited strong fluorescence, indicating successful genetic transformation. An intron was needed for the expression of egfp. The EGFP fluorescence intensities of the three transformants did not decay even after subculturing on uracil-containing semisolid medium 5 times, suggesting that the transformed plasmids were stably retained in the absence of selective pressure.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (4), 771-775, 2013

  公益社団法人 日本農芸化学会

キーワード

• <i>Cyathus stercoreus</i>
• biological pretreatment
• rice straw
• genetic transformation
• uracil auxotrophic mutant

詳細情報

• CRID: 1390001206477542784
• NII論文ID: 10031170717
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.120912
• COI: 1:STN:280:DC%2BC3srivFChsA%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024443169
• PubMed: 23563547
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024443169
• Web Site: https://www.tandfonline.com/doi/pdf/10.1271/bbb.120912
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可