Analysis of Chloroplast Genome of Two Cytoplasmic Male Sterile Lines Derived from Interspecific Chimera and Intergeneric Somatic Hybrid in Brassicaceae
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- Ohta Yuzo
- United Graduate School of Agriculture, Tokyo University of Agriculture and Technology
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- Hirata Yutaka
- Graduate School of Agriculture, Tokyo University of Agriculture and Technology
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- Motegi Takeshi
- Graduate School of Agriculture, Tokyo University of Agriculture and Technology
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- Hattori Gen
- United Graduate School of Agriculture, Tokyo University of Agriculture and Technology
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- Noguchi Takashi
- Graduate School of Agriculture, Tokyo University of Agriculture and Technology
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Two new types of cytoplasmic male sterile (CMS) lines have been developed, one by in vitro chimera formation between Brassica rapa and B. oleracea and another by protoplast fusion of B. oleracea with Raphanus sativus. These CMS lines harbored an Ogura-type mitochondrial gene, orf138. They expressed chlorosis at a young stage and their chloroplast genome changed to the type of Ogura radish based on restriction fragment length polymorphism (RFLP) analysis. Thus, we attempted to study the relationships among cytoplasmic male sterility, chlorosis and the variation of the chloroplast genome using the matK and RuBisCO genes. The two CMS lines, normal and male sterile radishes exhibited similar matK gene sequences, while B. rapa and B. oleracea showed matK sequences of the radish type in very low copy numbers. The CMS lines displayed an identical RFLP pattern with that of the male sterile radish in the RuBisCO gene region. These phenomena indicated that the chloroplast genome organization had changed from Brassica to Raphanus type or Raphanus CMS type by CMS induction, and suggested the existence of heteroplasia in the Brassica chloroplast.<br>
収録刊行物
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- Breeding Science
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Breeding Science 56 (1), 1-5, 2006
日本育種学会
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詳細情報 詳細情報について
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- CRID
- 1390001204722967808
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- NII論文ID
- 130004146046
- 110004361885
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- NII書誌ID
- AA11317194
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- ISSN
- 13473735
- 13447610
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- NDL書誌ID
- 7831049
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可