N2O induces mitotic polyploidization in anther somatic cells and restores fertility in sterile interspecific hybrid lilies

DOI Web Site Web Site PubMed 3 Citations 61 References
  • Nukui Shotarou
    The Graduate School of Science and Technology, Niigata University
  • Kitamura Satomi
    The Graduate School of Science and Technology, Niigata University Kaneko Seeds, Co., Ltd.
  • Hioki Tomoyo
    The Graduate School of Science and Technology, Niigata University
  • Ootsuka Hideaki
    Niigata Agricultural Research Institute Horticultural Research Center
  • Miyoshi Kazumitsu
    Faculty of Bioresources and Sciences, Akita Prefectural University
  • Satou Takao
    Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Agricultural Experiment Station
  • Takatori Yuka
    Saga Prefectural Agriculture Research Center
  • Oomiya Tomo
    Hokkaido Ornamental Plants and Vegetables Research Center
  • Okazaki Keiichi
    The Graduate School of Science and Technology, Niigata University

Search this article

Abstract

Fertile plants undergoing male gametogenesis can be treated with nitrous oxide (N2O) gas to obtain 2n male gametes. N2O treatment is also expected to restore the fertility of interspecific hybrids through meiotic restitution or mitotic amphidiploidization. However, this technique has few applications to date, and it is unknown how N2O treatment restores fertility in sterile hybrids. To establish optimal N2O treatment conditions and determine its cytological mechanism of action, we treated various sized floral buds with N2O gas at different anther developmental stages from fertile and sterile hybrid lilies. N2O treatment using the optimal 1–4 mm floral buds induced mitotic polyploidization of male archesporial cells to produce 2n pollen in fertile hybrid lilies. In sterile hybrid lilies, N2O treatment doubled the chromosome number in male archesporial cells followed by homologous chromosome pairing and normal meiosis in pollen mother cells (PMC), resulting in restoration of pollen fertility. Backcrossing the resultant fertile pollen to Lilium × formolongi produced many triploid BC1 plants. Thus N2O treatment at the archesporial cell proliferating stage effectively overcame pollen sterility in hybrid lilies, resulting in fertile, 2n pollen grains that could produce progeny. The procedure presented here will promote interspecific or interploidy hybridization of lilies.<br>

Journal

  • Breeding Science

    Breeding Science 61 (4), 327-337, 2011

    Japanese Society of Breeding

Citations (3)*help

See more

References(61)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top