Crosslinking of Cys-Mutated Human Galectin-1 to the Model Glycoprotein Ligands Asialofetuin and Laminin by Using a Photoactivatable Bifunctional Reagent
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- Tamura Mayumi
- Faculty of Pharmaceutical Sciences, Josai University
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- Watanabe Tomoe
- Faculty of Pharmaceutical Sciences, Josai University
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- Igarashi Takanori
- Department of Biological Chemistry, School of Pharmaceutical Sciences, Teikyo University
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- Takeuchi Tomoharu
- Faculty of Pharmaceutical Sciences, Josai University
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- Kasai Ken-ichi
- Department of Biological Chemistry, School of Pharmaceutical Sciences, Teikyo University
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- Arata Yoichiro
- Faculty of Pharmaceutical Sciences, Josai University
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抄録
Galectins are a group of animal lectins characterized by their specificity for β-galactosides. In our previous study, we showed that a human galectin-1 (hGal-1) mutant, in which a cysteine residue was introduced at Lys28, forms a covalently cross-linked complex with the model glycoprotein ligands asialofetuin and laminin by using the photoactivatable sulfhydryl reagent benzophenone-4-maleimide (BPM). In the present study, we used several hGal-1 mutants in which single cysteine residues were introduced at different positions and examined their ability to form a covalent complex with asialofetuin or laminin by using BPM. We found that the efficiency of formation of the cross-linked products differed depending on the positions of the cysteine introduced and also on the ligand used for crosslinking. Therefore, by using different cysteine hGal-1 mutants, the chances of isolating different ligands for hGal-1 should increase depending on the systems and cells used.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 37 (5), 877-882, 2014
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204632056704
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- NII論文ID
- 130004147328
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2cnpsVCktQ%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 025420024
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- PubMed
- 24790011
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可