Highlighted Paper selected by Editor-in-Chief : Stable Expression and Characterization of Monomeric and Dimeric Recombinant Hybrid-IgG/IgA Immunoglobulins Specific for Shiga Toxin
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- Iwata Koki
- Laboratory of Microbiology and Immunology, University of Shizuoka School of Pharmaceutical Sciences
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- Kurohane Kohta
- Laboratory of Microbiology and Immunology, University of Shizuoka School of Pharmaceutical Sciences
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- Nakanishi Katsuhiro
- Laboratory of Microbiology and Immunology, University of Shizuoka School of Pharmaceutical Sciences
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- Miyake Masaki
- Laboratory of Microbiology and Immunology, University of Shizuoka School of Pharmaceutical Sciences
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- Imai Yasuyuki
- Laboratory of Microbiology and Immunology, University of Shizuoka School of Pharmaceutical Sciences
書誌事項
- タイトル別名
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- Stable Expression and Characterization of Monomeric and Dimeric Recombinant Hybrid-IgG/IgA Immunoglobulins Specific for Shiga Toxin
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抄録
Antigen-specific immunoglobulin A (IgA) may be useful for preventing infectious diseases through passive immunization on the mucosal surface. We previously established mouse IgA and IgG monoclonal antibodies (mAbs) specific for the binding subunit of Shiga toxin 1 (Stx1B). We also developed a recombinant hybrid-IgG/IgA, in which variable regions from the IgG mAb were present. The binding activity of recombinant hybrid-IgG/IgA was verified by transient expression. Aiming at a constant supply, we established Chinese hamster ovary cells stably expressing monomeric or dimeric hybrid-IgG/IgA. The cDNAs encoding heavy and light chains were co-expressed for the monomeric hybrid-IgG/IgA, while those encoding heavy, light, and joining chains were co-expressed for the dimeric one. Serum-free culture supernatants of the cloned transfectants were subjected to size-exclusion chromatography. The elution patterns showed that the binding to immobilized Stx1B and the immunoblot signals of assembled immunoglobulins were correlated. In the transfectant for the dimeric hybrid-IgG/IgA, both monomers and dimers were observed. Size-exclusion chromatography enabled us to prepare a sample of the dimeric hybrid-IgG/IgA devoid of the monomeric one. The monomeric and dimeric forms of hybrid-IgG/IgA were prepared from the respective transfectants to examine the neutralization of Stx1. After pretreatment with monomeric or dimeric hybrid-IgG/IgA, the cytotoxicity of Stx1 toward Vero cells was abolished. Furthermore, the dimeric form was more than 10-fold more effective than the monomeric one in terms of toxin neutralization. These results suggest that the tetravalent feature of the binding sites of the dimeric hybrid-IgG/IgA contributes to the efficacy of toxin neutralization.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 37 (9), 1510-1515, 2014
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204634127488
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- NII論文ID
- 130004147362
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2cfpt1OitA%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 025736957
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- PubMed
- 24989136
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可