ＨＰＬＣポストカラム誘導体化蛍光法を用いた環境及び生物試料中のメソミル，オキサミルの分析

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  • Determination of Methomyl and Oxamyl in the Environmental and Biological Samples by HPLC with Post Column Fluorescence Derivatization.

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The analytical method of methomyl and oxamyl in environmental and biological samples has been developped by using the reverse phase high performance liquid chromatographic method equipped with post column fluorescence derivatization system.<BR>Both compounds in water samples were extracted with dichloromethane and the extracts were cleaned up by 5% hydrous silicagel mini-column chromatography.<BR>Both compound in sediment and fish samples were extracted with methanol and the extracts were diluted with 10% sodium chloride aqueous solution and washed with hexane. And then the extract was treated by the same operation on the water sample.<BR>A 20μL aliquot containing aldicarb-sulf oxide was injected into a reverse phase HPLC column. The analytes were hydrolyzed with 0.05N sodium hydroxide at 85°C. Methylamine produced by hydrolysis of oxamyl, methomyl and aldicarb-sulfoxide reacted with o-phthalaldehyde (OPA) and 2-mercaptoethanol to form a highly fluorescent derivative which was detected by a fluorescence detector.<BR>The present method was successfully applied to the analysis of methomyl and oxamyl in water, sediment and fish samples.

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  • 環境化学

    環境化学 3 (4), 771-782, 1993

    一般社団法人 日本環境化学会

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