Roles of Nitric Oxide Radicals in Intracellular Bactericidal Activities on Human Macrophage-like MABS-1 Cells
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- MATSUMOTO Michihiro
- <I>Department of Biochemistry, Showa University School of Medicine</I>
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- SUGISAKI Keizo
- <I>Department of Biochemistry, Showa University School of Medicine</I>
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- MATSUDA Isao
- <I>Department of Hematology, Showa University School of Medicine</I>
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- IWAMOTO Sanju
- <I>Department of Biochemistry, Showa University School of Medicine</I>
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- TAKEDA Minoru
- <I>Department of Biochemistry, Showa University School of Medicine</I>
抄録
We demonstrated examined the roles of specific free radical species on intracellular bactericidal activity of human macrophage-like MABS-1 cells. In an intracellular bactericidal activity assay in which MABS-1 cell phagocytosis and digestion of FITC-conjugated Escherichia coli (E. coli) is detected by FACScan®, almost all phagocytosed E. coli were killed within 3 h, whereas digestion of E. coli was delayed. Inducible nitric oxide synthase (iNOS) was expressed in MABS-1 cell cytosolic fractions, and iNOS inhibitors reduced the intracellular bactericidal activity. Generation of nitric oxide in MABS-1 cells was detected by diaminofluorescent-2 (DAF-2) assay, but not Griess assay, and responded to exposure to tumor necrosis factor and/or interferon-γ. Generation of peroxynitrite, which has strong cytotoxicity, was detected by dihydrorhodamine 123 assay, and NG-monomethyl-L-arginine, superoxide dismutase and nitrosobenzen, an inhibitor of NADPH oxidase, interfered with peroxynitrite generation. These results suggested that E. coli phagocytosed by MABS-1 cells were killed by nitric oxide (NO) radicals generated by iNOS, and that the killing activity was caused by peroxynitrite rather than NO.
収録刊行物
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- The Showa University Journal of Medical Sciences
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The Showa University Journal of Medical Sciences 11 (3), 161-170, 1999
昭和大学学士会
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詳細情報 詳細情報について
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- CRID
- 1390001204372933888
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- NII論文ID
- 130004189987
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- COI
- 1:CAS:528:DC%2BD3cXhvFGjsLs%3D
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- ISSN
- 21850968
- 09156380
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可