Alterations in Sarcoplasmic Reticulum and Mitochondrial Functions in Stunned Myocardium : Ralation between Regional Myocardial Function and Biochemical Analyses

• ARATA Hirohisa

  <I>Third Department of Internal Medicine, Showa University School of Medicine</I>

• GESHI Eiichi

  <I>Third Department of Internal Medicine, Showa University School of Medicine</I>

• ISHIOKA Haruhiko

  <I>Third Department of Internal Medicine, Showa University School of Medicine</I>

• KATAGIRI Takashi

  <I>Third Department of Internal Medicine, Showa University School of Medicine</I>

抄録

We studied the contribution of intracellular microorgan dysfunction in the post ischemic stunned myocardium. In 47 mongrel dogs, LAD occlusion for 15 min (I-15m) and subsequent reperfusion for 60 min (R-60m) were performed with simultaneous records of hemodynamics, coronary blood flow (CBF), regional myocardial blood flow (MBF) and segment shortening (SS) . Sarcoplasmic reticulum (SR) and mitochondria (Mt) were analyzed using biochemical procedures. Hemodynamics indicated no remarkable changes throughout the experiment. CBF showed reactive hyperemia to 370% of control transiently after reprefusion, then returned to control level at R-1 Sm. SS diminished to -22% of control at I-1 5m, and it was still depressed at 58% at R-60m (stunning) . Ca-ATPase activity of SR was reduced to 57% of control at I-15m, and remained depressed at 78% at R-60m. State III respiration of Mt decreased to 75% of control, and dinitrophenol-stimulated ATPase activity of Mt was 72% of control at I-15m. These activities did not recover completely at R-60m (77% and 75%, respectively) . Both Ca-ATPase activity of SR and state III respiration of Mt were positively correlated to SS. SDS-gel electrophoresis of SR protein revealed no irreversible changes. These results suggested that contractile dysfunction after brief ischemia is related to the energy demand and supply, and the transient dysfunction of microorgans is one of the main causes of stunned myocardium.

収録刊行物

• Ｔｈｅ　Ｓｈｏｗａ　Ｕｎｉｖｅｒｓｉｔｙ　Ｊｏｕｒｎａｌ　ｏｆ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅｓ

  Ｔｈｅ　Ｓｈｏｗａ　Ｕｎｉｖｅｒｓｉｔｙ　Ｊｏｕｒｎａｌ　ｏｆ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅｓ 7 (1), 83-94, 1995

  昭和大学学士会

キーワード

• stunned myocardium
• reperfusion injury
• sarcoplasmic reticulum
• mitochondria
• canine heart

詳細情報

• CRID: 1390282679350728192
• NII論文ID: 130004190292
• DOI: 10.15369/sujms1989.7.83
• ISSN: 21850968
• ISSN: 09156380
• Web Site: https://www.jstage.jst.go.jp/article/sujms1989/7/1/7_1_83/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可