Visualization of sulfated glycoconjugates in the chicken embryonic heart by whole mount alcian blue staining.
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- Wang Pei-Rong
- Department of Anatomy, Nihon University School of Dentistry
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- Isokawa Keitaro
- Department of Anatomy, Nihon University School of Dentistry Division of Electron Microscopy, Dental Research Center, Nihon University
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- Jiang Yi
- Department of Anatomy, Nihon University School of Dentistry
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- Sejima Hitomi
- Department of Anatomy, Nihon University School of Dentistry
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- Yokoyama Hirokazu
- Department of Anatomy, Nihon University School of Dentistry
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- Endo Seiichi
- Department of Anatomy, Nihon University School of Dentistry
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- Hama Itsuko
- Department of Anatomy, Nihon University School of Dentistry
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- Sugayama Taeko
- Department of Anatomy, Nihon University School of Dentistry
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- Toda Yoshihisa
- Department of Anatomy, Nihon University School of Dentistry Division of Electron Microscopy, Dental Research Center, Nihon University
抄録
In the atrioventricular canal (AVC) and outflow tract (OT) of the developing heart, endothelial cells transform specifically to mesenchymal cells. The mesenchymal cells migrate into the underlying acellular matrix termed cardiac jelly and form endocardial cushion tissue. It is believed the that the highly hydrated nature of cardiac jelly is ascribed to sulfated glycoconjugates in the components of jelly matrix. In the present study, we have visualized the distribution and its temporal changes of sulfated glycoconjugates in the embryonic heart from stage 12 to 26 using whole mount alcian blue (AB) histochemistry. Atrial matrix was AB-negative in all the stages examined. Cardiac jelly in the AVC and OT were positive and the staining intensity increased as heart development proceeded, while AB-positive staining in the matrix of the ventricle became negative by stage 19. At stages later than 19, AB-positive matrix was localized in only the AVC and OT where endothelially-derived mesenchymal cells populated. (J. Oral Sci. 40, 153-157, 1998)
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ
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Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ 40 (4), 153-157, 1998
日本大学歯学部
