活性化第ＸＩ因子‐α１アンチトリプシン複合体測定法
書誌事項
- タイトル別名
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- Quantification of activated factor XI-.ALPHA.1 antitrypsin complex by an enzyme-linked differential antibody immunosorbent assay.
抄録
We developed an assay for activated factor XI-α1antitrypsin complex (F. XIa-α1AT) in plasma. Purified F. XI was activated with β-XIIa and treated with α1AT. Thus we prepared the standard F. XIa-α1AT. Assay for F. XIa-α1AT was carried out essentially according to the method of Harpel. F. XIa-α1AT in sample plasma (10-fold dilution, 200μl) was adsorbed on anti-F. XI monoclonal antibody beads and developed with peroxidase-labeled anti-α1AT Fab'.<br>To eliminate the effect of plasma on this assay, standard F. XIa-α1AT was diluted with 10% F. XI-deficient plasma which did not contain the complex. The absorbance of the standard complex containing F. XI (0.016-0.12μg/assay, i. e. 20-150%) was a little lower than that of the complex alone, because F. XI compete the binding of F. XIa-α1AT to the beads. Either F. XI or α1AT alone did not increase the color development. Therefore we diluted the standard F. XIa-α1AT with 10% F. XI-deficient plasma containing F. XI (0.08μg/assay, i. e. 100%). Recovery of added F. XIa-α1AT was satisfactory (over 90%).<br>25 normal individuals had low level of F. XIa-α1AT (below 0.18ng/assay), whereas 30 patients with disseminated intravascular coagulation (DIC) demonstrated high level of this complex (0.18-4.2ng/assay). These data indicated that this assay was useful to the diagnosis of DIC.
収録刊行物
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- 血液と脈管
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血液と脈管 17 (3), 268-270, 1986
一般社団法人 日本血栓止血学会
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詳細情報 詳細情報について
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- CRID
- 1390001204858425216
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- NII論文ID
- 130004261149
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- ISSN
- 18842372
- 03869717
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
