Ｍｙｃｏｂａｃｔｅｒｉｕｍ ｔｕｂｅｒｃｕｌｏｓｉｓおよびＭｙｃｏｂａｃｔｅｒｉｕｍ ａｖｉｕｍ ｃｏｍｐｌｅｘに対するマクロファージの抗菌活性発現におよぼすＡＴＰの作用
書誌事項
- タイトル別名
-
- Effects of ATP on the in vitro and in vivo antimicrobial activity of murine macrophages against Mycobacterium avium complex.
抄録
Extracellular ATP stimulates a wide variety of cell types, including macrophages(MΦs), by ligating of plasma membrane purinergic receptors. Treatment of human MΦs with ATPΦ is reported to potentiate MΦ activity in killing Mycobacterium tuberculosis(MTB). We studied the effects of ATP on the in vitro and in vivo antimicrobial activity of murine MΦs against Mycobacterium avium complex(MAC). While ATP at concentration of 3-5mM up-regulated the antimicrobial activity of MΦs against MTB dose-dependently, this was not noted for MΦs infected with MAC organisms, although intramacrophage MAC growth was weakly inhibited in ATP-treated MΦs under certain conditions. When MAC-infected mice were subcutaneously given ATP at 40 mg/kg dose, once daily, 5 times per week, for up to 8 weeks after infection, bacterial growth in the lungs was inhibited significantly during the first 2 weeks compared to control mice without ATP administration. This effect on the intrapulmonary MAC growth was no longer observed after week 2, and MAC grew much more rapidly thereafter in mice given ATP than in the control mice. ATP didnot, however, affect MAC bacterial growth in the spleen. These findings show that ATP affects MTB antimicrobial activity against MAC negatively, in contrast to the positive effect against MTB. The finding that ATP administration inhibited MAC organism growth in the lungs of infected mice in the early stages of infection suggests that ATP may be useful in treating MAC infections when given in combination with anti-MAC antimicrobials.
収録刊行物
-
- 日本化学療法学会雑誌
-
日本化学療法学会雑誌 50 (12), 848-853, 2002
公益社団法人 日本化学療法学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206286820736
-
- NII論文ID
- 130004298092
-
- ISSN
- 18845886
- 13407007
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可