Detection of Herpes Simplex Virus (HSV) Infection by Using FITC-Labeled Monoclonal Antibodies (MicroTrak Method)

• KAWANA Takashi

  Department of Obstetrics and Gynecology, University of Tokyo

• KURATA Tsuyoshi

  Department of Pathology, The Institute of Medical Science, The University of Tokyo

• SATA Tetsutaro

  Department of Pathology, The Institute of Medical Science, The University of Tokyo

• KAWANA Rinji

  Department of Bacteriology, Iwate Medical University

• SATOH Seidai

  Department of Bacteriology, Iwate Medical University

• TAMAKI Kunihiko

  Department of Dermatology, University of Tokyo

• KUKITA Atsushi

  Department of Dermatology, University of Tokyo

• NIIMURA Michihito

  Department of Dermatology, Jikei University School of Medicine

• OHKI Yawara

  Department of Dermatology, Jikei University School of Medicine

• TEZUKA Tadashi

  Department of Dermatology, Kinki University School of Medicine

• YOSHIDA Masaki

  Department of Dermatology, Kinki University School of Medicine

• MORI Ryoichi

  Department of Virology, Kyushu University

• YASUMOTO Shinichiro

  Department of Virology, Kyushu University

Bibliographic Information

Other Title

• 蛍光標識モノクローナル抗体 (Microtrak Herpes) による単純ヘルペスウイルス感染症の診断
• ケイコウ ヒョウシキ モノクローナル コウタイ Microtrak Herpe

Abstract

The usefulness of FITC-labeled monoclonal antibodies against HSV-1 and HSV-2 (MicroTrak HSV-1/HSV-2 Direct Specimen Identification/Typing Test and Culture Confirmation Test) for the identification of fresh HSV isolates and for the detection of HSV infection in clinical specimens was studied.<BR>The Culture Confirmation Test identified 136 out of 137 known HSV-1 strains (99.4%) isolated in Japan as HSV-1 strains (99.4%) and all of 117 known HSV-2 strains as HSV-2.<BR>Ninety nine out of 122 (81.8%) clinical specimens obtained from culture proven herpetic lesions were determined as HSV-1 or HSV-2 infection by Direct Specimen Identification/Typing test.<BR>Sixteen clinical specimens obtained from culture negative lesions were positive for HSV antigen and were diagnosed as herpetic lesions.<BR>Though its sensitivity is somewhat lower than that of culture method, it gives results within 2 hours after specimen receipt, indicating that it is very useful for rapid diagnosis of HSV infection.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 61 (9), 1030-1037, 1987

  The Japanese Association for Infectious Diseases

Keywords

• Herpes simplex virus
• monoclonal antibody
• diagnosis

Details

• CRID: 1390001205048953984
• NII Article ID: 130004329327
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.61.1030
• ISSN: 1884569X
• ISSN: 03875911
• NDL BIB ID: 3160445
• PubMed: 2832486
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3160445
• Web Site: https://search.jamas.or.jp/link/ui/1988150470
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed