Ｈｂ中の単糖類の存在様式からみたＧｌｙｃｏｓｙｌａｔｅｄ　Ｈｂ分画測定の臨床的評価

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書誌事項

タイトル別名
  • Clinical Assessment of the Measurement of Percent HbA Fractions
  • Hbチュウ ノ タントウルイ ノ ソンザイ ヨウシキ カラ ミタ Glycos

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抄録

Hemoglobin AIa, b, c as components of the HbAI fraction, are known to arise by post-translational, non-enzymatic glycosylation of HbA at the β-chain-N-terminal amino group. Although HbAIa, b, c increase quantitatively in many cases with diabetes mellitus, the modes of attachment of monosaccharides to these subfractions remain unknown. We attempted to clarify this problem by utilizing a GC-MS system for monosaccharide analysis of glycosylated hemoglobins. The HbAIa, b, c and HbAo from normal controls, diabetics, and thyrotoxic patients with or without carbohydrate intolerance were fractionated by Bio-Rex 70 column chromatography, according to Trivelli's method. Each sample from the fractions of HbAIa, b, c and HbAo was treated to form TMSi monosaccharide derivatives, according to Bunn's method. Then, GC-MS (Shimadzu LKB 9000) mass fragmentography was performed to identify the monosaccharides. The following results were obtained.<BR>1) In normal controls, diabetics, and thyrotoxic patients, each fraction of HbAIa, b, c and HbAo was demonstrated to contain α-, β-mannose, glucose, and galactose. It seems likely therefore that glycosylation exists in HbAIa, b with several monosaccharides as it does in HbAIc but that HbAIa, b are at least glycosylated in different ways in both quantity and quality, so contributing to the differences in separation patterns of each of the HbAIa, b, c and HbAo. It is suggested that HbAIa, b may be in an intermediary position among the HbA which would be further glycosylated in HbAIc.<BR>2) As a whole, the percent HbAIc appears to represent a more significant index than that of HbAI (a+b+c) in reflecting the long-term state of glucose metabolism but individual cases are not always in accordance with this result.<BR>3) The hexose moieties of the HbAo fraction may show a relation with the percent HbAIc and percent HbAI (a+b+c) and also reflect the state of glucose metabolism.<BR>4) There was no correlation between the hexose contents of HbAo (or HbAI+HbAo) and the percent HbAI (a+b+c)(or percent HbAIc). The findings were at variance with previous results describing a significant correlation between the TBA colour value and percent HbAI (a+b+c).<BR>5) Qualitative and quantitative analyses of the sugar components of HbAI and HbAo may provide one means of elucidating the glucose metabolism.

収録刊行物

  • 糖尿病

    糖尿病 25 (5), 545-555, 1982

    一般社団法人 日本糖尿病学会

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