胆管癌の上皮内進展部における細胞増殖能，細胞活性およびｐ５３遺伝子産物の解析

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  • Analysis of Proliferative Activity and p53 Gene Product in the Intraepithelial Spread of Advanced Bile Duct Carcinoma.

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Introduction: Bile duct carcinoma is known to spread intraepithelially with high frequency, but biological features remain to be clarified. To differentiate clearly among biological features of the intraepithelial spread portion (IESP) and the invasive portion (IP), we studied the number of argyophilic nucleolar organizer region proteins (AgNOR), Ki-67 positive rate (Ki-67 PR), and p53 overexpression (p53 score). Methods: We stained 20 bile duct carcinoma specimens with antihuman p53 protein monoclonal antibody, antihuman Ki-67 protein monoclonal antibody by streptavidin-biotin immunoperoxidase, and AgNOR by Ploton's 1-step method. Results: Ki-67 PR was 21.5% in IESP and 20.2% in IP. AgNOR in IP was significantly larger than in IESP (IESP: 2.2, IP: 3.3, p=0.0012). Nuclear p53 immunopositivity was observed in 8 of 20 patients (40.0%) in IESP and 13 of 20 (65.0%) in IP. Cases of p53 positive stain in IP were also observed mostly in IESP. Conclusion: No difference in proliferative activity was seen between IESP and IP. This suggests that the cell kinetics of IESP are lower than in IP and that p53 overexpression plays a part in the early of carcinogenesis.

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