Streptococcus fecalisの變異に關する研究

• 杉原 久義

  東京大學傳染病研究所第一研究部 財團法人河野臨床醫學研究所

書誌事項

タイトル別名

• Studies on the Mutation of Streptococcus Fecalis

抄録

The mutation among 4 species of Enterococci, St. fecalis, St. liquefaciens, St. zymogenes and St. durans has not hitherto been experimentally demonstrated, although they are closely related to one another.<BR>The author could endow the capacity of β-hemolysis to St. fecalis, by means of successive transfer of the cocci together with a minimum amount of aseptic staphylotoxin in the testis of mice belonging to the dd-strain. This mutation was confirmed with 5 strains of St. fecalis, and the hemolysis thus induced was characteristic of that of the II-type of Kobayashi's classification (positive β-hemolysis both on horse and sheep blood). These 5 strains underwent mutation to the III-type of Kobayashi (β-hemolysis on horse blood, and α-hemolysis on sheep blood), when they were seeded on the “total heat sterilized fluid culture of original strain (St. fecalis) ”. The same mutation could also be induced in 4 of the 5 above strains within about a year, when the cocci belonging to the II-type were successively preserved on blood agar slants.<BR>These strains of II-and III-type showed resistence against antibiotics and following typical biological properties of enterococci. They grew in 0.1% methylen blue milk, 6.5% NaCl broth and in the broth at pH 9.6. They were furthermore relatively thermostable, resistant to potassium tellulite, could ferment Mannitol and Esculin and did not produce “soluble hemolysin”.<BR>The mutational strains were indistinguishable from the original strains by precipitation reaction and belonged likewise to the group-D of Lancefield.<BR>All the mutational II and III strains were therefore identified as St. zomogenes and the mutation of St. fecalis to St. zymogenes was thus demonstrated.<BR>According to Kobayashi, hemolytic enterococci should always belong to the III-type, but the author's experiment revealed their possible classification into the II-type. This must be taken into consideration in the application of the classification of Kobayashi.

収録刊行物

• 日本傳染病學會雜誌

  日本傳染病學會雜誌 30 (10), 902-917, 1957

  社団法人 日本感染症学会

詳細情報

• CRID: 1390282680071270144
• NII論文ID: 130004378741
• DOI: 10.11552/kansenshogakuzasshi1926.30.902
• ISSN: 18845681
• ISSN: 00214817
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可