内喉頭筋の酵素組織化学的研究

DOI
  • 笠 誠一
    Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University

書誌事項

タイトル別名
  • A Histochemical Study of Intrinsic Laryngeal Muscles

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抄録

Electromyographical studies demonstrate that the intrinsic laryngeal muscles contract in different grade and different phase during respiration, phonation and deglutition. In this investigation, the author tried to elucidate the relationship between the structure and the function of the canine laryngeal muscles by histochemical techniques.<BR>Thyroarytenoid, lateral cricoarytenoid, posterior cricoarytenoid, arytenoid and cricothyroid muscles were precisely removed and immediately frozen with aceton-dry ice solution. These muscles were sliced as the cross section of 8-10 micron thick in a cryostat (-20°C). Each serial section was stained with myofibrillar adenosine phosphatase, phosphorylase, reduced diphosphopyridine nucleotid dehydrogenase and succinic dehydrogenase respectively. Stained specimens were photographed in color and the distribution of various muscle fibers was histochemically observed.<BR>Histochemical property of the canine laryngeal muscle fibers could be divided into two types. The type I of muscle fiber showed low activity with myofibrillar adenosine triphosphatase and phosphorylase and high activity with reduced diphosphopyridine nucleotid dehydrogenase and succinic dehydrogenase. On the contrary, the type II of muscle fiber showed high activity with adenosine triphosphatase and phosphorylase and low activity with reduced diphosphopyridine nucleotid dehydrogenase and succinic dehydrogenase.<BR>The results of each muscle is as follows:<BR>1) Thyroarytenoid muscle; This muscle is usually divided into two parts, i. e. external part (M. lateralis) and internal part (M. vocalis). The maldistribution of muscle fibers is markedly observed in this muscle. The type I fiber is mostly distributed in the inner part of the muscle and gradually decreased and not found in the external part.(type I fiber =12.8%±6.02 SD)<BR>2) Lateral cricoarytenoid muscle; Both types of the muscle fibers are uniformly distributed at any part of the muscle, and amount of type II fiber is markedly dominant in general.(type I fiber =12.1%±5.26 SD)<BR>3) Posterior cricoarytenoid muscle; In this muscle, the distribution of type II fiber is slightly dominant.(type I fiber=39.8%±4.45 SD)<BR>4) Cricothyroid muscle; The distribution of both types of fibers of this muscle is resembled to that of the posterior cricoarytenoid muscle.(type I fiber =52.8%±13.75 SD)<BR>5) Arytenoid muscle; This muscle has also two types of muscle fibers.(type I fiber=9.6%±3.47 SD)<BR>On the physiological point of view, it is considered that the type I fiber is a slow twitch fiber and the type II fiber is a fast twitch fiber. Therefore, the mode or strength of the contraction of a whole muscle depends on the ratio of amount of these two types of muscle fibers. According to the physiological investigations, the contraction time of the intrinsic laryngeal muscle is longer in the thyroarytenoid, lateral cricoarytenoid, posterior thyroarytenoid and cricoarytenoid muscles in this order. This result corresponds to our histochemical observation on the distribution of type I fibers in a whole muscle.

収録刊行物

  • 耳鼻と臨床

    耳鼻と臨床 28 (2Supplement1), 394-402, 1982

    JIBI TO RINSHO KAI

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