Label-free Fluorescent Detection of Loop-mediated Isothermal Amplification of Nucleic Acid Using Pyrophosphate-selective Xanthene-based Zn(II)-coordination Chemosensor

• Ekkachai Kittiloespaisan

  Department of Biotechnology, Faculty of Science, Mahidol University

• Akio Ojida

  Graduate School of Pharmaceutical Sciences, Kyushu University

• Itaru Hamachi

  Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University

• Yortyot Seetang-Nun

  CENTEX Shrimp, Faculty of Science, Mahidol University

• Wansika Kiatpathomchai

  CENTEX Shrimp, Faculty of Science, Mahidol University

• Jirarut Wongkongkatep

  Department of Biotechnology, Faculty of Science, Mahidol University

抄録

A label-free fluorescent detection method for the loop-mediated isothermal amplification (LAMP) of nucleic acids was first developed by using the pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor 1–2Zn(II). In this method, the production of inorganic pyrophosphate (PPi), a by-product of the LAMP reaction, was detected by 1–2Zn(II) with a large fluorescence enhancement. On combination with the fluorescent plate reader technique, this method allows us to detect the shrimp white spot syndrome virus in a high-throughput manner with high sensitivity and selectivity.

収録刊行物

• Chemistry Letters

  Chemistry Letters 41 (12), 1666-1668, 2012-12

  Oxford University Press (OUP)

キーワード

• General Chemistry

詳細情報

• CRID: 1360283694088368896
• NII論文ID: 130004426481
• DOI: 10.1246/cl.2012.1666
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/41/12/1666/55640531/cl.2012.1666.pdf
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