発蛍光試薬によるDNA酸化損傷—5-ホルミル-2′-デオキシウリジン—選択的検出法の開発

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タイトル別名
  • Development of Selective Detection Method for 5-Formyl-2′-Deoxyuridine in DNA Using a Fluorogenic Reagent
  • 発蛍光試薬によるDNA酸化損傷 5-ホルミルー2'-デオキシウリジン 選択的検出法の開発
  • ハツ ケイコウ シヤク ニ ヨル DNA サンカ ソンショウ 5-ホルミルー 2'-デオキシウリジン センタクテキ ケンシュツホウ ノ カイハツ

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抄録

  It is important that various lesions in DNA were detected selectively and conveniently to know mechanisms of carcinogenicity and/or aging of cells. However, most detection methods of DNA lesion are complicated and take a long time for enzymatic hydrolysis and analysis by HPLC and/or mass spectrometry. This review shows the new concept for detection of DNA lesion by “fluorogenic reagent”. Inspired by the unique bis-heteroaryl structure of luciferin and 5-heteroaryl-2′-deoxyuridine having good fluorescence properties, we designed and synthesized fluorogenic reagent 4,5-dimethoxy-2-aminothiophenol for a selective and convenient detection for 5-formyl-2′-deoxyuridine, which is generated in yields comparable to that of 2′-deoxy-8-oxoguanosine, in DNA. Generated 5-(5,6-dimethoxybenzothiazol-2-yl)-2′-deoxyuridine has a high quantum yield and larger Stokes shift in aqueous solution. This derivatization of 5-formyl-2′-deoxyuridine in oligodeoxynucleotide occurred quickly and quantitatively. The fluorogenic reagent was also revealed to detect 5-formyl-2′-deoxyuridine in γ-irradiated calf thymus DNA with irradiation dose dependent manner. Thus, our fluorogenic strategy enables to selective and convenient detection of lesion in DNA exposed to various forms of oxidative stress.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 133 (10), 1041-1053, 2013-10-01

    公益社団法人 日本薬学会

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