Induction of Apoptotic Cell Death in HL-60 Cells by Jacaranda Seed Oil Derived Fatty Acids

• Yamasaki Masao

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Motonaga Chihiro

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Yokoyama Marino

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Ikezaki Aya

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Kakihara Tomoka

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Hayasegawa Rintaro

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Yamasaki Kaede

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Sakono Masanobu

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Sakakibara Yoichi

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Suiko Masahito

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

• Nishiyama Kazuo

  Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

抄録

Various fatty acids are attracting considerable interest for their anticancer effects. Among them, fatty acids containing conjugated double bonds show one of the most potent cytotoxic effects on cancer cells. Here, we focused on the cancer cell killing activity of jacaranda seed oil. The seed oil of jacaranda harvested from Miyazaki in Japan contained 30.9% cis-8, trans-10, cis-12 octadecatrienoic acid, called jacaric acid (JA). Fatty acid prepared from this oil (JFA) and JA strongly induced cell death in human leukemia HL-60 cells. On the other hand, linoleic acid and trans-10, cis-12 conjugated linoleic acid (<10 μM) did not affect cell proliferation and viability. An increase in the sub-G₁ population and internucleosomal fragmentation of DNA was observed in JA- and JFA-treated cells, indicating induction of apoptotic cell death. Finally, the cytotoxic effects of JA and JFA were completely abolished by α-tocopherol. Taken together, these data suggest that jacaranda seed oil has potent apoptotic activity in HL-60 cells through induction of oxidative stress.

収録刊行物

• Journal of Oleo Science

  Journal of Oleo Science 62 (11), 925-932, 2013

  公益社団法人 日本油化学会

キーワード

• jacaric acid
• jacaranda seed oil
• apoptosis
• oxidative stress

詳細情報

• CRID: 1390282679068893056
• NII論文ID: 130004436004
• NII論文ID: 40019836642
• NII書誌ID: AA11503337
• DOI: 10.5650/jos.62.925
• COI: 1:STN:280:DC%2BC2c7jvFWjug%3D%3D
• ISSN: 13473352
• ISSN: 13458957
• NDL書誌ID: 024947557
• PubMed: 24200941
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024947557
• Web Site: https://www.jstage.jst.go.jp/article/jos/62/11/62_925/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014136490
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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