Process Optimization of Enzyme Catalyzed Production of Dietary Diacylglycerol (DAG) Using TLIM as Biocatalyst
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- Dhara Rupali
- Food Engineering and Technology Department, Institute of Chemical Technology
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- Singhal Rekha S.
- Food Engineering and Technology Department, Institute of Chemical Technology
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Diacylglycerol (DAG)-rich sunflower oil was prepared and the optimal conditions for synthesis of DAG-rich oil by glycerolysis using biocatalyst TLIM was determined. A maximum production of 59.8% DAG was obtained after 5 h of constant reaction under vacuum (756 mm of Hg). The optimum temperature for glycerolysis was found to be 50°C, while stoichiometric molar ratio of sunflower oil:glycerol was 2:1 for this reaction. A minimum acid value of 0.48 mg of KOH.g–1 of oil was observed under these conditions. The fatty acid composition of DAG-rich oil was found to be similar to the original TAG-rich sunflower oil used in the work. The lipase catalysed glycerolysis using 1,3 specific lipase was used to promote the formation of 1,3 isoform of DAG as this isoform is known to possess anti-obesity effect. DAG content was determined by HPTLC and GCMS. The DAG-rich oil contained 59.75% DAG of which 63.34% was found as 1,3-DAG and 36.65% was 1,2-DAG/2,3-DAG.
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 63 (2), 169-176, 2014
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204091924992
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- NII論文ID
- 130004436106
- 40019952634
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- NII書誌ID
- AA11503337
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- COI
- 1:STN:280:DC%2BC2cvisF2ktA%3D%3D
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 025165719
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- PubMed
- 24500104
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可