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- Pedro Prudencio B.
- Laboratory of Animal Science, College of Agriculture, Kochi University
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- 桜井 孝志
- 高知大学農学部
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- 枝重 圭祐
- 高知大学農学部
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- 葛西 孫三郎
- 高知大学農学部
書誌事項
- タイトル別名
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- Effects of Osmotic Shrinkage on the Survival of Mouse Oocytes and Embryos at Various Developmental Stages.
- Effects of Osmotic Shrinkage on the Sur
この論文をさがす
抄録
To examine the sensitivity of mammalian oocytes and embryos to osmotic shrinkage, which can occur during the process of removal of cryoprotectant from cryopreserved cells, the effect of shrinkage on the survival of fresh and vitrified mouse oocytes at metaphase II and embryos at 1-cell to blastocyst was examined. Oocytes and embryos were suspended in PBS media containing various concentrations of sucrose for 30 min at 25°C. They were then returned to isotonic PBS, and the survival was assessed in vitro. Fresh oocytes and embryos were almost entirely insensitive to shrinkage in a solution with 0.75 M sucrose, but in solutions with 1.0-1.5 M sucrose, oocytes and 8-cell embryos were more sensitive to the hypertonic stresses, whereas 2-cell embryos and expanded blastocysts were less sensitive. Vitrified embryos were more sensitive to hypertonic stresses than were fresh ones, but the sensitivity was reduced when the embryos had been cultured for a short period before subjecting them to the hypertonic stress.<br>
収録刊行物
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- 日本哺乳動物卵子学会誌
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日本哺乳動物卵子学会誌 14 (1), 66-71, 1997
日本卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390001204339034880
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- NII論文ID
- 10013833933
- 130004436546
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- NII書誌ID
- AN10548943
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- ISSN
- 13475878
- 13417738
- http://id.crossref.org/issn/09167625
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- NDL書誌ID
- 4206791
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可