Reactivity of Ferrocenylcarbodiimide with DNA Duplex Containing Single-mismatched Base Pairs

MUKUMOTO Kosuke, WATANABE Sadayoshi, NOJIMA Takahiko, WAKI Michinori, TAKENAKA Shigeori

doi:10.2116/analsci.23.645

Reactivity of Ferrocenylcarbodiimide with DNA Duplex Containing Single-mismatched Base Pairs

DOI PDF Web Site Web Site Web Site 参考文献13件

MUKUMOTO Kosuke

Department of Applied Chemistry, Faculty of Engineering, Kyushu University
WATANABE Sadayoshi

Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology
NOJIMA Takahiko

Department of Applied Chemistry, Faculty of Engineering, Kyushu University
WAKI Michinori

Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology
TAKENAKA Shigeori

Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology

この論文をさがす

抄録

Ferrocenylcarbodiimide (1), which is known to react with a guanine (G) or thymine (T) base of single stranded DNA, was allowed to react with DNA duplex having a single mismatched base pair of G-T, T-T, or T-cytosine (C). Electrophoreograms of the reaction mixture showed that 1 could react with G or T base of the mismatched sites on the DNA duplex. However, 1 also reacted with the G base of the terminal site on the DNA duplex. This showed that 1 can react with an unpaired base or unstable base pair such as a terminal or mismatched base on the DNA duplex. Electrochemical mismatch detection could be achieved after hybridization of the ferrocenylated mismatched DNA duplex with a selected DNA probe-immobilized electrode. These results revealed that 1 has a potentiality of serving as a labeling reagent of mismatched bases on the DNA duplex, which is important in the search for heterozygous single nucleotide polymorphisms (SNPs).