Interference-free Determination of Indole-3-Acetic Acid in Two Real Systems Using Second-order Calibration Method Coupled with Excitation-emission Matrix Fluorescence

• YAN Xiu-Fang

  State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University

• WU Hai-Long

  State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University

• QING Xiang-Dong

  State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University

• SUN Yan-Mei

  State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University

• YU Ru-Qin

  State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University

抄録

In this work, a simple and practicable method that combines excitation-emission matrix (EEMs) fluorescence with a second-order calibration method based on parallel factor analysis-alternative least-squares (PARAFAC-ALS) algorithm was developed for the direct interference-free determination of indole-3-acetic acid (IAA) in two real systems, coconut water (CW) and coconut milk (CM). Although the excitation and emission profiles of IAA heavily overlapped with that of unknown interferents in the complex real systems, the actual contents and satisfactory recoveries were still obtained successfully. The contents of IAA in CW and CM were 10.8 ± 0.3 and 4.9 ± 0.2 μg mL⁻¹, respectively, which were consistent with those reported by LC-MS/MS assays in the reference material. The average spike recoveries of IAA in the validation set based on CW and CM were 102.1 ± 3.2 and 98.0 ± 1.9%, respectively. In addition, routine experiments were performed for establishing the validity of the assay to internationally accepted criteria.

収録刊行物

• Analytical Sciences

  Analytical Sciences 30 (4), 489-494, 2014

  社団法人 日本分析化学会

キーワード

• IAA
• EEMs
• second-order calibration
• PARAFAC-ALS

詳細情報

• CRID: 1390001204260077056
• NII論文ID: 130004442278
• NII論文ID: 40020036875
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.30.489
• COI: 1:STN:280:DC%2BC2cnitlWktg%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 025389858
• PubMed: 24717659
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025389858
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.30.489.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.30.489/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/30/4/30_489/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可