Conophylline and Betacellulin-.DELTA.4: an Effective Combination of Differentiation Factors for Pancreatic .BETA. Cells

• KITAMURA Ryu-ichi

  Institute for Molecular and Cellular Regulation, Gunma University Third Department of Internal Medicine, National Defense Medical College

• OGATA Takeki

  Institute for Molecular and Cellular Regulation, Gunma University Third Department of Internal Medicine, National Defense Medical College

• TANAKA Yuji

  Third Department of Internal Medicine, National Defense Medical College

• MOTOYOSHI Kazuo

  Third Department of Internal Medicine, National Defense Medical College

• SENO Masaharu

  Department of Bioscience and Technology, Graduate School of Bioscience and Biotechnology, Okayama University Research Center for Biomedical Engineering, Okayama University

• TAKEI Izumi

  Clinical Laboratory and Internal Medicine, School of Medicine Keio University

• UMEZAWA Kazuo

  Department of Applied Chemistry, Faculty of Science and Technology, Keio University

• KOJIMA Itaru

  Institute for Molecular and Cellular Regulation, Gunma University

抄録

Conophylline and betacellulin-δ4 reproduce differentiation-inducing activity of activin A and betacellulin, respectively. We examined the effect of conophylline and betacellulin-δ4 on β cell differentiation. In AR42J cells, conophylline and betacellulin-δ4 converted them into insulin-producing cells. Cells treated with conophylline and betacellulin-δ4 continued to grow after differentiation. Thus, cell number and insulin content were much greater compared to cells treated with activin A and betacellulin. Furthermore, cells treated with conophylline and betacellulin-δ4 secreted insulin in response to glucose. Likewise, conophylline and betacellulin-δ4 converted pancreatic ductal cells into insulin-producing cells. Insulin content, cell number and glucose-evoked insulin secretion were significantly greater than those in cells treated with activin A and betacellulin. Transplantation of pseudoislets prepared using ductal cells treated with conophylline and betacellulin-δ4 was able to reduce effectively the plasma glucose concentration in streptozotocin-treated nude mice. Conophylline and betacellulin-δ4 are effective in inducing differentiation of β cells from progenitors.<br>

収録刊行物

• Endocrine Journal

  Endocrine Journal 54 (2), 255-264, 2007

  一般社団法人 日本内分泌学会

キーワード

• Pancreatic β cell
• Insulin
• Differentiation
• Progenitor

詳細情報

• CRID: 1390282681274155264
• NII論文ID: 130004443291
• DOI: 10.1507/endocrj.k06-199
• ISSN: 13484540
• ISSN: 09188959
• Web Site: http://www.jstage.jst.go.jp/article/endocrj/54/2/54_2_255/_pdf
• 本文言語コード: en
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