Selective Culture Method for Hepatocyte-like Cells Differentiated from Human Induced Pluripotent Stem Cells
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- KONDO Yuki
- Department of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University
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- YOSHIHASHI Sachimi
- Educational Research Center for Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Nagoya City University
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- MIMORI Kayo
- Educational Research Center for Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Nagoya City University
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- OGIHARA Ruri
- Educational Research Center for Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Nagoya City University
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- KANEHAMA Yoshinori
- Primary Cell Division, COSMO BIO Co., Ltd.
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- MAKI Yoshiyuki
- Primary Cell Division, COSMO BIO Co., Ltd.
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- ENOSAWA Shin
- Division for Advanced Medical Services, National Center for Child Health and Development
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- KUROSE Kouichi
- Division of Medicinal Safety Science, National Institute of Health Sciences Department of Food Science and Technology, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology
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- IWAO Takahiro
- Department of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University Educational Research Center for Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Nagoya City University
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- NAKAMURA Katsunori
- Department of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University Educational Research Center for Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Nagoya City University
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- MATSUNAGA Tamihide
- Department of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University Educational Research Center for Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Nagoya City University
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抄録
This study aimed to establish culture conditions which are able to give the differentiation of induced pluripotent (iPS) cells to hepatocytes. To this end, we examined the usefulness of a culture medium containing the components involved in the intermediary metabolism in the liver. More specifically, we examined the effect of the “modified L-15 medium” containing galactose, phenylalanine and ornitine, but deprived of glucose, tyrosine, arginine and pyruvic acid. The medium was altered according to changes in the expression of enzymes that participate in liver-specific pathways. After 25 days of differentiation, the differentiated cells expressed hepatocyte markers and drug-metabolizing enzymes. These expression levels were increased using modified L-15 medium. The survival of human fetal liver cells and the death of human fibroblasts were observed during culture in modified L-15 medium. Most of the cells that differentiated from human iPS cells using modified L-15 medium were stained by anti-human albumin antibody. These results suggest that iPS cells can be converted to high purity-differentiated hepatocytes by cultivating them in modified L-15 medium.
収録刊行物
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- Ｄｒｕｇ Ｍｅｔａｂｏｌｉｓｍ ａｎｄ Ｐｈａｒｍａｃｏｋｉｎｅｔｉｃｓ
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Ｄｒｕｇ Ｍｅｔａｂｏｌｉｓｍ ａｎｄ Ｐｈａｒｍａｃｏｋｉｎｅｔｉｃｓ 29 (5), 407-413, 2014
日本薬物動態学会