Periodontal tissue regeneration by transplantation of rat adipose-derivedstromal cells in combination with PLGA-based solid scaffolds
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- AKITA Daisuke
- Nihon University Graduate School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan
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- MOROKUMA Masakazu
- Department of PartialDenture Prosthodontics, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan
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- SAITO Yoko
- Departmentof Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan
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- YAMANAKA Katsuyuki
- GC corp.R&D, 76-1 Hasunumacho, Itabashi-ku Tokyo 171-8585, Japan
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- AKIYAMA Yuko
- Nihon University Graduate School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan
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- SATO Momoko
- Nihon University Graduate School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan
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- MASHIMO Takayuki
- Nihon University Graduate School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan
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- TORIUMI Taku
- Nihon University Graduate School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan
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- ARAI Yoshinori
- Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-kuTokyo 101-8310, Japan
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- KANEKO Tadashi
- GC corp.R&D, 76-1 Hasunumacho, Itabashi-ku Tokyo 171-8585, Japan
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- TSUKIMURA Naoki
- Department of PartialDenture Prosthodontics, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-kuTokyo 101-8310, Japan
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- ISOKAWA Keitaro
- Department of Anatomy, Nihon University School of Dentistry, 1-8-13Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan; 6 Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-kuTokyo 101-8310, Japan Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-kuTokyo 101-8310, Japan
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- ISHIGAMI Tomohiko
- Department of Anatomy, Nihon University School of Dentistry, 1-8-13Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan; 6 Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-kuTokyo 101-8310, Japan Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-kuTokyo 101-8310, Japan
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- HOND Masaki J
- Department of Anatomy, Nihon University School of Dentistry, 1-8-13Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan; 6 Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-kuTokyo 101-8310, Japan Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-kuTokyo 101-8310, Japan
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抄録
Regeneration of damaged periodontium is challenging due to its multi-tissue composition. Mesenchymalstem cell-based approaches using adipose-derived stromal cells (ASCs) may contribute toperiodontal reconstruction, particularly when combined with the use of scaffolds to maintain aspace for new tissue growth. The aim of this study was to assess the regenerative potential ofASCs derived from inbred or outbred rats in combination with novel solid scaffolds composed ofPLGA (Poly d,l-lactic-co-glycolic acid) (PLGA-scaffolds). Cultured ASCs seeded onto PLGAscaffolds(ASCs/PLGA) or PLGA-scaffolds (PLGA) alone were transplanted into periodontal fenestrationdefects created in F344 or Sprague Dawley (SD) rats. Micro-CT analysis showed asignificantly higher percentage of bone growth in the ASCs/PLGA groups compared with thePLGA-alone groups at five weeks after surgery. Similarly, histomorphometric analysis demonstratedthicker growth of periodontal ligament and cementum layers in the ASCs/PLGA-groups comparedwith the PLGA-alone groups. In addition, transplanted DiI-labeled ASCs were observed inthe periodontal regenerative sites. The present investigation demonstrated the marked ability ofASCs in combination with PLGA scaffolds to repair periodontal defects.
収録刊行物
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- Biomedical Research
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Biomedical Research 35 (2), 91-103, 2014
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