書誌事項
- タイトル別名
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- Further Structural Study of the Barley and Bamboo Shoot Xyloglucans by Xyloglucanase
抄録
To investigate the arrangement of the glucose residues which are not xylosylated at O-6 in the β-1,4-D-glucan backbone of xyloglucan from Gramineae, a xyloglucan from immature barley plants was hydrolyzed by a purified Geotrichum sp. M128 xyloglucan specific endo-1,4-β-D-glucanase (xyloglucanase). The fragment oligosaccharides were then analyzed by matrix-assisted laser-desorption ionization time of the flight mass spectrometry before and after treatment with an oligoxyloglucan-specific glycosidase, oligoxyloglucan reducing end-specific cellobiohydrolase from Geotrichum sp. M128, with a mixture of isoprimeverose-producing oligoxyloglucan hydrolase from Eupenicillium sp. M9 and β-D-glucosidase from Trichoderma viride, or with β-D-galactosidase from Bacillus circulans. Tetra-, penta-, hexa-, hepta-, octa- and nona-saccharide fractions were obtained as major oligosaccharide fractions, which might be composed of XX, of XXG, of XXGG, of XXGGG and LXGG, of XLGGG, LXGGG, XXGGGG and GXXGGG, and of XLGGGG, LXGGGG, GXLGGG, GLXGGG and XXGGGGG, respectively. In addition, the oligosaccharide units of bamboo shoot xyloglucan were analyzed. The structure of bamboo shoot xyloglucan was shown to be very similar to that of barley xyloglucan. These results suggest that the Gramineae xyloglucan has regions consisting of two, three, four and five contiguous glucose residues which are not xylosylated at O-6 in the β-1,4-D-glucan backbone.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 51 (4), 327-333, 2004
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390282681270398208
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- NII論文ID
- 130004480839
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- COI
- 1:CAS:528:DC%2BD2cXhtVCnt7vI
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- ISSN
- 18807291
- 13447882
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可