アリル特異的ＰＣＲ法に用いられるプライマーの検討

• 渡邉 剛太郎

  山形県警察本部刑事部科学捜査研究所

• 梅津 和夫

  山形大学医学部法医病態診断学分野

書誌事項

タイトル別名

• Examination of the Primeres for Allele-Specific PCR Amplification

抄録

  Many methods for analysis of SNP based on using the allele-specific amplification technique were reported. In these methods, we must use the highly allele-specific primers. However, the specificity of primer depends on the type of SNP and the nucleotide sequence. In this study, we examined the relation between the specificity of primer and the oligonucleotide sequence for typing of NY95. The NY95 typing is based on a A-T transversion mutation. Results showed that the allele-specific primers which have the 3′-terminal nucleotide substitution and the miss-matched nucleotide at 3-5th position from 3′-terminal were highly specific. Furthermore, when the method in which the opposed allele-specific primers were used in one tube, non-specific amplification were not observed.<br>   Consequently, it seems that the opposed allele-specific primers and the non-specific amplification inhibit each other.<br>

収録刊行物

• 日本法科学技術学会誌

  日本法科学技術学会誌 12 (1), 121-125, 2007

  日本法科学技術学会

キーワード

• Allele-specific primer
• SNPs typing
• PCR amplification

詳細情報

• CRID: 1390001205245326976
• NII論文ID: 130004485320
• DOI: 10.3408/jafst.12.121
• ISSN: 18814689
• ISSN: 18801323
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可