高分子結合性ペプチドを用いた機能性ソフト界面の創製

• 伊達 隆明

  東京大学先端科学技術研究センター

• 芹澤 武

  東京大学先端科学技術研究センター

書誌事項

タイトル別名

• Fabrication of Functional Soft Interfaces Using Polymer-Binding Peptides

抄録

We demonstrated the selection and characterization of polymer-binding peptides, and their application as surface modifiers. Peptides that bind specifically to polyetherimide (PEI) films were successfully identified from a phage-displayed peptide library. A chemically-synthesized peptide with the sequence of Thr-Gly-Ala-Asp-Leu-Asn-Thr (p1) showed high affinity toward PEI films (5.6×10⁸ M^-1). The peptide was biotinylated and immobilized onto the PEI films to function as a linker for the subsequent immobilization of streptavidin (SAv). We found that several factors could significantly affect the amount of SAv bound. Furthermore, we demonstrated that the SAv immobilized onto the PEI films via the biotinylated peptides was uniformly distributed, and thus was able to further immobilize probe DNA and to observe hybridization with complementary DNA. The present study offered new and significant insights into the modification of polymer film surfaces with specific binding peptides.

収録刊行物

• 表面科学

  表面科学 33 (1), 21-26, 2012

  公益社団法人 日本表面科学会

キーワード

• soft interface
• surface modification
• polymer-binding peptide
• polyetherimide

詳細情報

• CRID: 1390001206457057024
• NII論文ID: 130004486695
• DOI: 10.1380/jsssj.33.21
• ISSN: 18814743
• ISSN: 03885321
• Web Site: http://www.jstage.jst.go.jp/article/jsssj/33/1/33_1_21/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可