Protoplasting, Regeneration and Transformation of Rare Actinomycetes.

• Zhang Hui

  Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University

• Shinkawa Hidenori

  Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University

• Kinashi Haruyasu

  Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University

• Hasegawa Toru

  Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University

抄録

Seventeen species of 11 rare actinomycete genera were treated with lysozyme and achromopeptidase to produce protoplasts. Fourteen of them could be protoplasted. Abundant protoplasts were obtained from Actinokineospora globicatena, Actinosynnema mirum and Amycolatopsis azurea, while relatively small amount of protoplasts were obtained from two Actinomadura species and Couchioplanes caeruleus subsp. azureus. Nine of 14 protoplasted species could regenerate on R1M and R2YE media with the range of regeneration frequency. The protoplasts of Amycolatopsis azurea regenerated on R1M and R2YE at the highest frequency (80%). Actinomadura atramentaria, A. echinospora, Catellatospora ferruginea and two Couchioplanes species could not regenerate on any tested media. A. azurea could be transformed with a Streptomyces vector, pIJ702, at the frequency of 1.6 x 10⁶ transformants per μg DNA. The other Streptomyces plasmid pRES18 was also able to be maintained in this strain.

収録刊行物

• 日本放線菌学会誌

  日本放線菌学会誌 13 (1), 42-48, 1999

  日本放線菌学会

キーワード

• General Medicine

詳細情報

• CRID: 1390282679677677440
• NII論文ID: 130004487492
• NII論文ID: 10008263361
• NII書誌ID: AN10080091
• DOI: 10.3209/saj.13_42
• ISSN: 18816371
• ISSN: 09145818
• NDL書誌ID: 4822767
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4822767
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可