Characterization of the dnaK Locus in Streptomyces griseus.

Inada Kuninobu, Kinashi Haruyasu, Nimi Osamu, Hasegawa Toru, Shinkawa Hidenori

doi:10.3209/saj.16_37

Characterization of the dnaK Locus in Streptomyces griseus.

DOI Web Site 参考文献18件

Inada Kuninobu

Department of Fermentation Technology, Graduate School of Engineering, Hiroshima University
Kinashi Haruyasu

Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
Nimi Osamu

Hiroshima Bunkyo Women’s College
Hasegawa Toru

Department of Food and Nutrition, Sonoda Women’s University
Shinkawa Hidenori

Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

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The nucleotide sequence of the dnaK locus in Streptomyces griseus 2247 revealed a gene organization consisting of 6 genes (5’-orf1-dnaK-grpE-dnaJ-hspR-orf6-3’). The genes orf1 and orf6 were predicted to transcribe in the direction opposite that of dnaK, grpE, dnaJ and hspR. This locus of S. griseus was compared with that of S. coelicolor A3(2). No homolog of orf1 and orf6 was found in S. coelicolor, while dnaK, grpE, dnaJ and hspR were highly similar to those of S. coelicolor. Promoter probing analysis revealed significant promoter activity in the upstream region of the dnaK gene. The interspace regions of contiguous genes (dnaK-grpE-dnaJ-hspR) were amplified by RT-PCR of mRNA, indicating that these four genes constitute a polycistronic operon. His-tagged HspR protein was overexpressed in Escherichia coli and purified.