THE EFFECTS OF ULTRAVIOLET IRRADIATION AND HYPOXIA ON EXPRESSION OF GLUTATHIONE PEROXIDASE AND GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE IN THE CULTURED RETINAL PIGMENT EPITHELIUM

• AKEO Kiyoshi

  Akeo Eye Clinic Department of Ophthalmology, Keio University School of Medicine

• FUNAYAMA Tomoo

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency

• HAMADA Nobuyuki

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine

• AKEO Yoko

  Akeo Eye Clinic

• HIRAMITSU Tadahisa

  Photon Medical Research Center, Hamamatsu University, School of Medicine

• KOBAYASHI Yasuhiko

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine

抄録

Glutathione peroxidase (GPX) protects the phospholipids in the biomembrane barrier from damage by the free radicals. We studied cultured bovine retinal pigmented epithelial (RPE) cells exposed to UV-A and UV-B to try to determine the expression of GPX and glyceraldehdye-3-phosphate dehydrogenase (GAPDH) that are highly sensitive to oxidation. The total cellular RNA of the RPE cells was reverse transcribed by using primers for GPX and GAPDH. The quantitative real-time RT-PCR was performed to compare the effects of UV-A and UV-B irradiation on 18S ribosomal RNA (rRNA) and GPX expression using the LightCycler system. The expression of GAPDH and GPX was decreased by hyperoxia such as 20% oxygen and UV-B irradiation using electrophoresis. The LightCycler analysis, the quantitative real-time RT-PCR, showed that UV-A exposure at a small dose induced the defense mechanism against lipid peroxidation in RPE cells. However, UV-A exposure at a large dose led to a disturbance in the defense mechanism similar to that observed with UV-B irradiation; this could have a relationship with retinal diseases caused by light damage.

収録刊行物

• 組織培養研究

  組織培養研究 26 (3), 149-157, 2007

  日本組織培養学会

キーワード

• Retinal pigment epithelium
• glutathione peroxidase
• glyceraldehyde 3-phosphate dehydrogenase

詳細情報

• CRID: 1390001204920324864
• NII論文ID: 130004495275
• NII論文ID: 10019965031
• NII書誌ID: AA11048252
• DOI: 10.11418/jtca.26.149
• ISSN: 18813704
• ISSN: 09123636
• Web Site: https://search.jamas.or.jp/link/ui/2008103011
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可