過酸化水素／低温プラズマ放電滅菌法の作用機構に関する研究―指標菌の脂質の構造変化―

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書誌事項

タイトル別名
  • Study of Sterilizing Mechanism of the Plasma Discharging Technique in the Presence of Hydrogen Peroxide at Lower Temperature-Characteristic Chane of Lipid in Bucilli-
  • カサンカスイソ/テイオン プラズマ ホウデン メッキンホウ ノ サヨウ キコウ ニ カンスル ケンキュウ : シヒョウキン ノ シシツ ノ コウゾウ ヘンカ
  • -Characteristic Chane of Lipid in Bucilli-
  • -指標菌の脂質の構造変化-

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An attempt to observe the characteristic change of total lipid in B. stearothermophilus and of B. atrophaes by the plasma discharge sterilizing technique in the presence of hydrogen peroxide vapor at lower temperature has carried out.<BR>At first step, 10mL of B.stearothermophilus and of B.atrophaeus incubated solution (107/mL) in glass vial tube were divided to 5mL X 2 tubes and were weighed, respectively. These samples were dried up by the centrifugal freezing technique at reduced pressure. One of these bacilli were sterilized (Sterilized sample) and another tube was stood (Non sterilized sample, Blank). Total lipid of these bacilli was extracted by the Folch's solvent (chloroform, 2 vol./methanol, 1 vol.). After this mixed solvent was also removed by the above mentioned dry up method, and all residue of these bacilli was treated by methanolisis. These esterified samples were analyzed by gas chromatography.<BR>It is observed that the main component of the extracted lipid of these bacilli is linolate (C18 : 2) and that B. stearothemophilus is more sensitive than B. atrophaeus on the resistance against this sterilizing technique.

収録刊行物

  • 医療機器学

    医療機器学 82 (1), 49-54, 2012

    一般社団法人日本医療機器学会

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