Tissue Distribution of Intravenously Administered Insoluble Microparticles: Report II

• Sugahara Takuya

  Department of Pharmacy, Yamagata City Hospital Saiseikan

• Kotani Masaharu

  Department of Molecular and Cellular Biology, School of Pharmaceutical Science, Ohu University

• Kuramoto Keiji

  Laboratory of Clinical Pharmaceutics, School of Pharmaceutical Science, Ohu University

• Arai Kouichi

  Department of Pharmacy, Yamagata City Hospital Saiseikan

• Shiraishi Tadashi

  Division of Hospital Pharmacy, Yamagata University

• Shoji Tohru

  Laboratory of Clinical Pharmaceutics, School of Pharmaceutical Science, Ohu University

抄録

In this study, we investigated the in vivo distribution of insoluble fluorescent microparticles administered to rats through the central vein. We previously reported that the IMs increased by mixed operation of some injections. The number of 10-25 μm insoluble microparticles was 41 ± 1 /mL. Therefore, the daily 10-μm fluorescent microparticle dose was set at 2000/day for the nutritional solution dose of 50 mL/day required to maintain the rats. The fluorescent microparticles were administered by bolus injection into the central vein on Days 1, 2, and 3. A fluorescence microscope was used to assess frozen thin sections of each organ that were prepared on Day 4 and urine samples. A large number of fluorescent microparticles were found in the lung tissue, with 1-5 particles in each of the 20-μm thick and 25-mm² area sections. In 20-μm thick and 50-mm² area kidney tissue sections, the number of observed fluorescent microparticles ranged from 0.1 to 0.2. No fluorescent microparticles were detected in tissue samples from the veins, cerebrum, cerebellum, heart, or liver. The total number of fluorescent microparticles excreted in the urine was 283.4 ± 113.0, which only accounted for approximately 4.7% of the dose administered over the 3-day period. These findings suggest that 10 μm insoluble microparticles administered intravenously, accumulate mainly in the lungs and kidneys and are excreted at low levels in the urine.

収録刊行物

• 医療薬学

  医療薬学 38 (9), 559-567, 2012

  一般社団法人日本医療薬学会

キーワード

• insoluble microparticles
• tissue distribution
• admixture
• accumulation
• excretion in urine

詳細情報

• CRID: 1390001204775238400
• NII論文ID: 130004502768
• DOI: 10.5649/jjphcs.38.559
• ISSN: 18821499
• ISSN: 1346342X
• Web Site: https://search.jamas.or.jp/link/ui/2013107470
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可