奄美諸島在来カンキツ遺伝資源のアイソザイムおよび DNA 分析

• 山本 雅史

  鹿児島大学農学部

• 河野 留美子

  鹿児島大学農学部

• 中川 剛志

  鹿児島大学農学部

• 臼井 拓磨

  鹿児島大学農学部

• 久保 達也

  鹿児島大学農学部

• 冨永 茂人

  鹿児島大学農学部

書誌事項

タイトル別名

• Isozyme and DNA Analyses of Local Citrus Germplasm on Amami Islands, Japan
• Isozyme and DNA analyses of local <italic>Citrus</italic> germplasm on Amami Islands, Japan

抄録

Genetic relationships among local Citrus accessions on the Amami islands, Japan, were evaluated using isozyme, random amplified polymorphic DNA (RAPD) and cleaved amplified polymorphic sequence (CAPS) of chloroplast DNA (cpDNA) analyses. Four loci were detected for three enzymes examined in isozyme analysis. Four and three kinds of genotypes were detected in glutamate oxaloacetate transminase (GOT)-1 and GOT-2, respectively. At least six genotypes were observed in peroxidase (PX). All accessions showed the same genotype except for one accession of superoxide dismutase (SOD)-3. In general, accessions that belong to the same species or type showed identical genotypes; however, some diversity of genotypes was observed within the same species and type. On the basis of the RAPD data, genetic relationships were assessed using NJ cluster analysis. From this result, accessions could be classified into three major clusters, A, B, and C. Cluster A included C. nobilis (Kunenbo), C. keraji (‘Keraji-Kikaijima’ and Kabuchii), C. oto (Oto), ‘Keraji-Kakeromajima’, and ‘Oto-Okinoerabujima’. Cluster B included only control accessions. Cluster C could be divided into four subclusters as follows: subcluster C. depressa (Shiikuwasha), some Sour orange relatives such as ‘Fusuu’ and ‘Kusa’, ‘Kikaimikan-Okinoerabujima’, and ‘Shimamikan’. Accessions were classified into three types in cpDNA analysis. Type I included C. nobilis (Kunenbo), C. keraji (‘Keraji-Kikaijima’ and Kabuchii), C. oto (Oto), ‘Keraji-Kakeromajima’, ‘Oto-Okinoerabujima’, and C. rokugatsu. Type II was composed of C. depressa (Shiikuwasha), ‘Kikaimikan-Okinoerabujima’, ‘Kusa’, and ‘Shiikuu’. Type III consisted of only ‘Shimamikan’. All mandarin accessions that were determined to belong to cluster A in RAPD analysis were included in type I of cpDNA analysis. Meanwhile, mandarins in types II and III in cpDNA analysis consisted of accessions in cluster C of RAPD analysis.<br>

収録刊行物

• Journal of the Japanese Society for Horticultural Science

  Journal of the Japanese Society for Horticultural Science 80 (3), 268-275, 2011

  一般社団法人　園芸学会

キーワード

• CAPS
• cpDNA
• genetic resources
• RAPD
• Ryukyu (Nansei) islands

詳細情報

• CRID: 1390001205289006592
• NII論文ID: 40018904630
• NII論文ID: 130004510719
• NII書誌ID: AA12177046
• DOI: 10.2503/jjshs1.80.268
• ISSN: 1882336X
• ISSN: 18823351
• HANDLE: 10232/18908
• NDL書誌ID: 11156060
• Web Site: https://ir.kagoshima-u.ac.jp/records/5564
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11156060
• Web Site: http://www.jstage.jst.go.jp/article/jjshs1/80/3/80_3_268/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可