PCR Primers for Marker Assisted Backcrossing to Introduce a CTV Resistance Gene from Poncirus trifoliata (L.) Raf. into Citrus
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- Ohta Satoshi
- Faculty of Agriculture, Shizuoka University Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS
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- Endo Tomoko
- Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS
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- Shimada Takehiko
- Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS
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- Fujii Hiroshi
- Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS
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- Shimizu Tokuro
- Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS
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- Kuniga Takeshi
- Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS
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- Yoshioka Terutaka
- Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS
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- Nesumi Hirohisa
- Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS
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- Yoshida Toshio
- Okitsu Citrus Research Station, National Institute of Fruit Tree Science, NIFTS
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- Omura Mitsuo
- Faculty of Agriculture, Shizuoka University
Bibliographic Information
- Other Title
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- カラタチ由来の CTV 抵抗性遺伝子をカンキツ属に戻し交雑で導入するために役立つ DNA マーカーセット
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Abstract
Citrus tristeza virus (CTV) is an acute pathogen that causes serious damage to the citrus industry. Poncirus trifoliata (L.) Raf., a sexually compatible species with Citrus, has resistance against a broad range of CTV strains. Breeding programs have been conducted to introduce the CTV resistance gene from P. trifoliata to Citrus, but no commercial cultivar has yet been developed. In this study, we developed four selection markers linked to CTV resistance to enable marker assisted selection to efficiently introduce CTV resistance into Citrus. The four CTV resistance-linked markers were composed of a co-dominant single nucleotide polymorphism (SNP) marker and three dominant sequence tagged site (STS) markers. All four markers were fitted to the progeny and were linked to CTV resistance, with only 2.8% exceptions. We also developed 46 P. trifoliata allele identification markers from alleles of 35 Citrus species. Among the 46 markers, nine were located in linkage group 2, on which the CTV resistance locus is located. We located the other 31 markers on the rest of the linkage groups so that these markers could be used to distinguish P. trifoliata genome regions remaining in the hybrid progenies. The set of PCR primers developed in this study will be useful for marker assisted backcrossing to introduce the P. trifoliata CTV resistance gene into Citrus.<br>
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 80 (3), 295-307, 2011
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390282680265745664
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- NII Article ID
- 130004510722
- 40018904633
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- NII Book ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- NDL BIB ID
- 11156142
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed