トレニア（<i>Torenia fournieri</i> Lind. ex Fourn.）の新規変異体「雀斑（そばかす）」は <i>R2R3-MYB</i> 遺伝子への DNA 型トランスポゾンの挿入によって花が斑入りになる
書誌事項
- タイトル別名
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- A Torenia (<i>Torenia fournieri</i> Lind. ex Fourn.) Novel Mutant ‘Flecked’ Produces Variegated Flowers by Insertion of a DNA Transposon into an <i>R2R3-MYB</i> Gene
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抄録
A novel torenia (Torenia fournieri Lind. ex Fourn.) mutant ‘flecked’, which bears variegated flowers, was obtained from ethyl methanesulfonate-treated M2 plants. The lower lip of this mutant has small violet spots with a pale violet background, while that of the normal type is solid violet. The mutant trait frequently reverted to a semicircular violet sector or solid violet lower lip. Germinal revertant plants with a solid violet lower lip also frequently occurred in S1 plants derived from self-pollinated mutant flowers. In the lower lip of the mutant type, anthocyanin concentration was much lower than in the normal type. This was attributed to decreased expression of the genes encoding anthocyanin biosynthesis enzymes, i.e. torenia chalcone synthase (TfCHS), flavanone 3-hydroxylase (TfF3H), dihydroflavonol 4-reductase (TfDFR), anthocyanidin synthase (TfANS), and UDP-glucose 3-O-flavonoid glucosyltransferase (TfUFGT). In the lower lip of the mutant, expression of a gene encoding R2R3-MYB transcription factor (TfMYB1, Torenia fournieri MYB1) was much lower than in the normal type and the revertants; this was caused by insertion of a Enhancer/Suppressor-Mutator (En/Spm)-like transposon (Ttf1, Transposon Torenia fournieri 1) in the 2nd intron of TfMYB1. Furthermore, it was found that the reversion of anthocyanin accumulation in the lower lips correlated to excision of Ttf1 from the TfMYB1. Overexpression of TfMYB1 in torenia caused anthocyanins to accumulate in the purple callus as a result of enhanced expression of the five structural genes mentioned above, demonstrating that TfMYB1 regulates these genes. Therefore, we concluded that a homozygous allele of Ttf1-inserted TfMYB1 caused the mutant phenotype. Ttf1 is a non-autonomous element because Ttf1 does not have the DNA sequence encoding transposase. Based on these results, potential uses of the flecked mutant for torenia breeding and transposon tagging are discussed.<br>
収録刊行物
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 82 (1), 39-50, 2013
一般社団法人 園芸学会
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詳細情報 詳細情報について
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- CRID
- 1390001205290768512
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- NII論文ID
- 130004510764
- 40019554349
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- NII書誌ID
- AA12177046
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- COI
- 1:CAS:528:DC%2BC3sXitl2itbY%3D
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- ISSN
- 1882336X
- 18823351
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- NDL書誌ID
- 024212512
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
