A comparison of two methods of obtaining densities of zooxanthellae in Acropora millepora

• M. MCCOWAN Dominique.

  ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville School of Marine and Tropical Biology, James Cook University, Townsville

• S. PRATCHETT Morgan

  ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville

• S. PALEY Alison

  ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville School of Marine and Tropical Biology, James Cook University, Townsville

• SEELEY Michelle

  ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville

• H. BAIRD Andrew

  ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville

書誌事項

タイトル別名

• A comparison of two methods of obtaining densities of zooxanthellae in <I>Acropora millepora</I>

抄録

Quantification of zooxanthellae densities in tissues of reef-building corals aids in the assessment of the extent and severity of coral bleaching. Various meth-ods are available to quantify zooxanthellae densities; how-ever, a direct comparison of these techniques has yet to been done. Here, we compare estimates of zooxanthellae densities obtained using conventional airbrushing coupled with post-tissue-blasting surface area determination, ver-sus a technique whereby zooxanthellae densities are quantified from a known area (0.25 cm²) of tissue after corals have been fixed and decalcified. Estimates of zoo-xanthellae densities obtained were correlated across re-plicate colonies (R²=0.40, n=81), and both techniques revealed similar patterns of variation among locations. The main benefit of the decalcification technique was reduced processing time, because the technique eliminates the time-consuming process of tissue blasting and re-trospective estimates of surface area. We estimate that decalcification halves the processing time per sample, and produces a more accurate estimate of zooxanthellae density.

収録刊行物

• Galaxea, Journal of Coral Reef Studies

  Galaxea, Journal of Coral Reef Studies 13 (1), 29-34, 2011

  日本サンゴ礁学会

キーワード

• Bleaching
• Coral reefs
• Decalcification tech-nique
• Zooxanthellae densities

詳細情報

• CRID: 1390001205301148032
• NII論文ID: 130004543588
• DOI: 10.3755/galaxea.13.29
• ISSN: 18833969
• ISSN: 18830838
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可