Construction of a Novel Single Double-Conditional shRNA Expression Vector
-
- MATSUOKA Tomoyuki
- Department of Pharmacology, Showa University School of Medicine
-
- OYAMADA Hideto
- Department of Pharmacology, Showa University School of Medicine
-
- NAKANO Masahideo
- Department of Pharmacology, Showa University School of Medicine
-
- SOMEI Masayuki
- Department of Pharmacology, Showa University School of Medicine
-
- TOMOYORI Eiji
- Department of Pharmacology, Showa University School of Medicine
-
- KONDO Yasuyuki
- Department of Pharmacology, Showa University School of Medicine
-
- OGUCHI Katsuji
- Department of Pharmacology, Showa University School of Medicine
この論文をさがす
抄録
Silencing of gene expression by RNA interference (RNAi) has become a widely used tool for assessing gene function in a fast and easy manner. An important advance in the RNAi field was the discovery that plasmid-based short hairpin RNA transcription can substitute for synthetic small interference RNAs both in vitro and in vivo. The constitutive knockdown of gene expression by RNAi can limit the scope of experiments, especially if the inhibition of genes leads to cell lethality, which prevents in vivo functional analysis. We have generated double-conditional short hairpin RNA from a single vector that can regulate its own transcription by a combination of tetracycline-inducible and Cre-loxP systems. This vector will be useful for the control of short hairpin RNA expression in a spatially-regulated manner by Cre recombinase as well as a temporally-regulated manner by tetracycline induction. These control features provide for the specific silencing of genes to facilitate functional genetic analysis in mammals in vivo.
収録刊行物
-
- The Showa University Journal of Medical Sciences
-
The Showa University Journal of Medical Sciences 25 (1), 19-27, 2013
昭和大学学士会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679350840320
-
- NII論文ID
- 130004566580
-
- NII書誌ID
- AA10781651
-
- COI
- 1:CAS:528:DC%2BC3sXhvVWgtrrO
-
- ISSN
- 21850968
- 09156380
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- IRDB
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可