Enzyme-lmmunoassay of Human α<SUB>1</SUB>-Microglobulin

• TAKAGI KIMITEMU

  Department of Clinical Pathology, Jichi Medical School

• KOYAMAISHI YOSHIHIRO

  Fujizoki Pharmaceutical Company

• ITOH YOSHIHISA

  Department of Clinical Pathology, Jichi Medical School

• ENOMOTO HIROMITSU

  Department of Clinical Pathology, Jichi Medical School

• KAWAI TADASHI

  Department of Clinical Pathology, Jichi Medical School

抄録

A simple and sensitive enzyme-immunoassay technique for quantitation of human α₁-microglobulin was established for the first time.The sandwich method, using anti-α₁-microglobulin goat immunogiobulin G (lgG)-coated polystyrene beads and horseradish peroxidase-labeled antibody, was employed for immunoassay.Small polystyrene beads were loaded with goat antibody lgG using a simple adsorption technique.This method is highly sensitvie and is capable of detecting minimum concentration of α₁-microglobulin in body fluids of 0.5ng/ml.The recovery rates of α₁-microglobulin in sera and urine were 97.8% and 98.9%, respectively, and α₁-microglobulin determinations on serial dilutions of both samples showed a satisfactory linearity.The coefficierlts of within-assay variation ranged between 2.8-6.2%in sera and 2.3-7.9%in urine, and those of between-assay variation 1.8-4.9%in sera and 1.3-6.0%in urine.α1-microglobulin leveis in the sera of 60normal adults quantitated in this study were found to be 8.82±2.9mg/l (mean±1SD), ranging from 5.89 to 11-75mg/l, While those in random Urines were 1.78±0.90mg/l, ranging from 0.69 to 3.92mg/l.This enzyme-immunoassay method should prove useful determining levels of α1-microglobulin in body fluids in which it might be present only in extremely low levels, and, moreover, is suitable for routine use in clinical laboratories.

収録刊行物

• Japanese Journal of Clinical Chemistry

  Japanese Journal of Clinical Chemistry 10 (1), 30-39, 1981

  一般社団法人 日本臨床化学会

詳細情報

• CRID: 1390001204911742080
• NII論文ID: 130004626997
• DOI: 10.14921/jscc1971b.10.1_30
• COI: 1:CAS:528:DyaL3MXlsVKmsb0%3D
• ISSN: 21874077
• ISSN: 03705633
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可