ダンシルクロライドを用いた蛍光観察による角質厚み測定
書誌事項
- タイトル別名
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- The Measurement of thickness of stratum corneum using dansyl chloride fluorescence
抄録
We measured intensity and spectrum of DC fluorescence in order to measure thickness of stratum corneum. Five % DC ointment was applied occlusively for 24 hrs on lower abdominal skin of mongrel dogs, we stripped the skin in several grades with adhesive tape, and measured DC fluorescence with He-Cd laser radiation (325nm) and UV lamp radiation (366nm) using photomultipliers. We employed normalization method using one of UV lamp emission line, the normalized DC fluorescence declined depending on the stripping rate. We also studied the enhance effects for tape stripping by kelatolytic agent (5% salicylic acid). Although stratum corneum was clearly decreased in histological obserbation, we could not obtain clear dependence of DC fluorescence on corneum thickness by some issues on histlogical specimen preparation
収録刊行物
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- 日本レーザー医学会誌
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日本レーザー医学会誌 16 (Supplement), 289-292, 1995
Japan Society for Laser Surgery and Medicine
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詳細情報 詳細情報について
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- CRID
- 1390282680432278400
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- NII論文ID
- 130004630451
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- ISSN
- 02886200
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可