ダンシルクロライドを用いた蛍光観察による角質厚み測定

DOI

書誌事項

タイトル別名
  • The Measurement of thickness of stratum corneum using dansyl chloride fluorescence

抄録

We measured intensity and spectrum of DC fluorescence in order to measure thickness of stratum corneum. Five % DC ointment was applied occlusively for 24 hrs on lower abdominal skin of mongrel dogs, we stripped the skin in several grades with adhesive tape, and measured DC fluorescence with He-Cd laser radiation (325nm) and UV lamp radiation (366nm) using photomultipliers. We employed normalization method using one of UV lamp emission line, the normalized DC fluorescence declined depending on the stripping rate. We also studied the enhance effects for tape stripping by kelatolytic agent (5% salicylic acid). Although stratum corneum was clearly decreased in histological obserbation, we could not obtain clear dependence of DC fluorescence on corneum thickness by some issues on histlogical specimen preparation

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282680432278400
  • NII論文ID
    130004630451
  • DOI
    10.2530/jslsm1980.16.supplement_289
  • ISSN
    02886200
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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