Intracellular calcium ion concentrations in endothelial cells in preeclampsia

• Matsubara Keiichi

  Department of Obstetrics and Gynecology, Ehime University School of Medicine

• Mori Miki

  Department of Obstetrics and Gynecology, Ehime University School of Medicine

• Matsubara Yuko

  Department of Obstetrics and Gynecology, Ehime University School of Medicine

• Uchikura Yuka

  Department of Obstetrics and Gynecology, Ehime University School of Medicine

• Nawa Akihiro

  Department of Obstetrics and Gynecology, Ehime University School of Medicine

• Oka Keizo

  Department of Integrated Center for Science, Ehime University School of Medicine

抄録

Aim: Preeclampsia (PE) is a disorder characterized by hypertensive symptoms and proteinuria in pregnant women. Previous studies have demonstrated that endothelial cell (EC) dysfunction is involved in the pathogenesis of PE. Intracellular free calcium ions are thought to function as secondary messengers in the EC signaling pathway. The aim of this study is to evaluate factors found in sera of PE patients by measuring their effects on intracellular calcium ion concentration ([Ca²⁺]i) in ECs.<br>Methods: ECs obtained from umbilical cords of normal pregnant women were cultured and incubated with Fura-2/acetoxymethyl ester (Fura-2AM). [Ca²⁺]i values were measured in ECs treated with sera from 29 normal pregnant women, 7 PE patients, and 10 non-pregnant women by monitoring fluorescence using a CAM 220 fluorometer.<br>Results: [Ca²⁺]i values were significantly higher in ECs treated with sera from normal pregnant women compared to those from non-pregnant women, and the effects were even stronger when ECs were treated with sera from pregnant women who were in later stages of pregnancy. Furthermore, sera from PE patients significantly increased EC [Ca²⁺]i compared to those from normal pregnant women in the 3rd trimester.<br>Conclusions: It is possible that serum factors play important roles in the maintenance of normal pregnancy. Furthermore, EC activation could be associated with the pathogenesis of PE through increases in EC [Ca²⁺]i.

収録刊行物

• Hypertension Research in Pregnancy

  Hypertension Research in Pregnancy 1 (2), 93-97, 2013

  日本妊娠高血圧学会

キーワード

• Ca²⁺
• C18 column
• endothelial cell
• preeclampsia
• serum

詳細情報

• CRID: 1390282680485681280
• NII論文ID: 130004638244
• DOI: 10.14390/jsshp.1.93
• ISSN: 21879931
• ISSN: 21875987
• Web Site: https://www.jstage.jst.go.jp/article/jsshp/1/2/1_93/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015032762
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
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