The Patterns of Labeling of Plasma Cells and Pyroninophilic Blast Cells with Tritiated Deoxycytidine and Thymidine

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  • OSOGOE BUNSUKE
    Department of Anatomy, Kawasaki Medical School, Matsushima, Kurashiki
  • MIYAMOTO HAJIME
    Department of Anatomy, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto

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  • Patterns of Labeling of Plasma Cells an

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By the autoradiographic techniques using 3H-deoxycytidine (3HdCyd)and 3H-thymidine (3HdThd), the patterns of labeling of plasma cells and pyroninophilic blast cells (immunoblasts), which are proliferating in response to antigenic stimulation, were studied in comparison with the labeling patterns of germinal-center cells.<BR>The majority of plasma cells and immunoblasts were much more heavily labeled with 3HdThd than were the germinal-center cells. On the contrary, the ability to utilize 3HdCyd of the former two cell types was definitely inferior to that of the latter cell type. However, either plasma cells or immunoblasts were intermingled with a smaller number of cells which were as intensely labeled with 3HdCyd as were the germinal-center cells. Such cells are most likely the progenitors of plasma cells or immunoblasts.

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